| Strain, strain background (mouse) |
Reeler mouse (B6C3Fe-ala-Relnrl) |
Jackson Laboratory |
Strain 000235 |
Used for OEC mouse tissue sections cultures, and western blots |
| Strain, strain background (rat) |
GFP-expressing Sprague-Dawley rats (SD-Tg) |
SLC, Shizuoka, Japan7 |
Z-004 SD-Tg (Act-EGFP) |
Used for breeding |
| Strain, strain background (rat) |
Unlabeled Sprague-Dawley female rats (SD) |
Charles Rivers Laboratory |
Strain 001 CD IGS |
Used for breeding with SD-Tg males |
| Strain, strain background (rat) |
GFP-SD-Tg males bred with SD females |
Sources noted above |
Used to derive 8- to 10-week-old female GFP-SD-Tg rats |
OECs used for scRNA-Seq analysis and rat cultures |
| Antibody |
Atf3; Mouse monoclonal |
Abcam |
Cat# ab191513, RRID:AB_2868437
|
1:4000; TSA + citric acid |
| Antibody |
Aif1/Iba1, Rabbit polyclonal |
Wako (Osaka, Japan) |
Cat# 019-19741, RRID:AB_839504
|
1:5000; IF |
| Antibody |
Anxa3, Rabbit polyclonal |
Gene Tex |
Cat# GTX103330, RRID:AB_11174712
|
1:5000; TSA + citric acid |
| Antibody |
Apolipoprotein E (Apoe), Goat polyclonal |
Sigma-Aldrich |
Cat# AB947, RRID:AB_2258475
|
1:2000; TSA + citric acid |
| Antibody |
Brain lipid-binding protein (Blbp), Rabbit monoclonal |
Sigma-Aldrich |
Cat# ZRB13190, RRID:AB_2920766
|
1:5000; IF |
| Antibody |
Brain lipid-binding protein (Blbp), Rabbit polyclonal |
Sigma-Aldrich |
Cat# ABN14, RRID:AB_10000325
|
1:1500; IF |
| Antibody |
Connective tissue growth factor (Ctgf, Ccn2), Rabbit polyclonal |
Abcam |
Cat# ab6992, RRID:AB_305688
|
1:500, IF |
| Antibody |
Gliomedin (Gldn), Rabbit polyclonal |
Abcam |
Cat# ab24483, RRID:AB_2111616
|
1:100; IF + citric acid |
| Antibody |
Growth-associated protein (Gap43), Rabbit polyclonal |
Abcam |
Cat# ab16053, RRID:AB_443303
|
1:500; IF |
| Antibody |
Green fluorescent protein (GFP), Chicken polyclonal |
Aves Labs Inc |
Cat# GFP-1020, RRID:AB_10000240
|
1:1000; IF |
| Antibody |
Glial fibrillary acidic protein (GFAP), Mouse monoclonal |
BD Biosciences |
Cat# 556327, RRID:AB_396365
|
1000; IF |
| Antibody |
Glial fibrillary acidic protein (GFAP), goat polyclonal |
Sigma |
Cat# SAB2500462, RRID:AB_10603437
|
1:2000; IF |
| Antibody |
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Mouse monoclonal |
Sigma-Aldrich |
Cat# MAB374, RRID:AB_2107445
|
1:100K; WB |
| Antibody |
L1, Rabbit polyclonal |
Gift from Dr. V Lemmon Brittis et al., 1995
|
|
1:10,000; IF |
| Antibody |
Marker of proliferation ki-67 (Mki67), Rabbit polyclonal |
Abcam |
Cat# ab15580, RRID:AB_443209
|
1:500; IF 1:5000; TSA |
| Antibody |
Nestin (Nes), Mouse monoclonal |
Developmental Studies Hybridoma Bank |
Cat# Rat-401, RRID:AB_2235915
|
1:1000; TSA + citric acid |
| Antibody |
Neurofilament-200 (NF), Rabbit polyclonal |
Sigma-Aldrich |
Cat# AB1989, RRID:AB_91202
|
1000; IF |
| Antibody |
N-Cadherins, Mouse monoclonal |
Invitrogen |
Cat# 33-3900, RRID:AB_2313779
|
1:500; IF |
| Antibody |
Neural cell adhesion molecule (NCAM), Rabbit polyclonal |
Sigma |
Cat# AB5032, RRID:AB_2291692
|
1:5000; IF |
| Antibody |
Nerve growth factor receptor, p75 (Ngfrp75), Mouse monoclonal |
Hybridoma gift from Dr. EM Shooter (Chandler et al., 1984) |
192-IgG |
1:50; IF 1:5; immuno-purification for OECs |
| Antibody |
Nerve growth factor receptor, p75 (Ngfrp75), Rabbit polyclonal |
Sigma-Aldrich |
Cat# AB1554, RRID:AB_90760
|
1:30K; IF 1:1500; immuno-purification |
| Antibody |
Peripheral myelin protein 22 (Pmp22), Rabbit polyclonal |
Gene Tex |
Cat# GTX85834, RRID:AB_10733560
|
1:1000; TSA + MeOH* |
| Antibody |
Reelin (Reln), Goat polyclonal |
R&D Systems |
Cat# AF3820, RRID:AB_2253745
|
1:250; IF |
| Antibody |
Reelin (Reln), Mouse monoclonal G10 |
Sigma-Aldrich |
Cat# MAB5364, RRID:AB_2179313
|
1:1000; F 1:750; WB |
| Antibody |
S100 calcium-binding protein β (S100β), Rabbit polyclonal |
Dako A/S (Glostrup, Denmark) |
Cat# GA50461-2, RRID:AB_2811056
|
1:500; IF |
| Antibody |
Serotonin (5HT), Goat polyclonal |
Immunostar |
Cat# 20079, RRID:AB_572262
|
5000; IF |
| Antibody |
Stathmin 1 (Stmn1), Rabbit monoclonal |
Abcam |
Cat# ab52630, RRID:AB_2197257
|
1:7000; TSA + citric acid |
| Antibody |
SRY-Box transcription factor 10 (SOX10), Goat polyclonal |
R&D Systems |
Cat# AF2864, RRID:AB_442208
|
1:75; ICC |
| Antibody |
Ubiquitin-conjugating enzyme E2 C (Ube2c), Rabbit monoclonal |
Abcam |
Cat# ab252940, RRID:AB_2910263
|
1:1000; TSA + citric acid |
| Antibody |
Alexa Fluor 488 AffiniPure donkey anti-Chicken IgY |
Jackson Immuno Research |
Cat# 703-545-155, RRID:AB_2340375
|
1:200–500 |
| Antibody |
Alexa Fluor 488 AffiniPure donkey anti-goat |
Jackson Immuno Research |
Cat# 705-545-003, RRID:AB_2340428
|
1:250 |
| Antibody |
Alexa Fluor 488 AffiniPure donkey anti-mouse |
Jackson Immuno Research |
Cat# 715-545-150, RRID:AB_2340846
|
1:500 |
| Antibody |
Alexa Fluor 488 donkey anti-rabbit |
Invitrogen |
Cat# A21206, RRID:AB_2535792
|
1:250 |
| Antibody |
Alexa Fluor 555 donkey anti-mouse |
Life Technologies |
Cat# A31570 RRID:AB_2536180
|
1:200–500 |
| Antibody |
Alexa Fluor 555 donkey anti-rabbit |
Life Technologies |
Cat# A31572, RRID:AB_162543
|
1:500 |
| Antibody |
Alexa Fluor 647 donkey anti-rabbit |
Jackson Immuno Research |
Cat# 711-605-152, RRID:AB_2492288
|
1:200–500 |
| Antibody |
Biotin-SP AffiniPure donkey anti-mouse IgG |
Jackson Immuno Research |
Cat# 715-065-150, RRID:AB_2307438
|
1:1000 |
| Antibody |
Biotin-SP AffiniPure donkey anti-rabbit IgG |
Jackson Immuno Research |
Cat# 711-065-152, RRID:AB_2340593
|
1:1000 |
| Antibody |
Biotin-SP AffiniPure goat anti-rabbit IgG, FC fragment specific |
Jackson Immuno Research |
Cat#111-065-008, RRID:AB_2337959
|
1:1000 hydrophobic Petri dishes |
| Antibody |
Biotinylated horse anti-goat |
Vector Laboratories |
Cat# BA-9500, RRID:AB_2336123
|
1:1000 |
| Other |
Streptavidin-conjugated horseradish peroxidase |
PerkinElmer |
Cat# NEL750001ET, RRID:AB_2617185
|
1:1000 |
| Commercial assay or kit |
Tyramide Signal Amplification (TSA) plus Fluorescein |
PerkinElmer |
Cat# NEL741001KT |
1:150 |
| Commercial assay or kit |
Tyramide Signal Amplification (TSA) plus Cyanine 3 |
PerkinElmer |
Cat# NEL74400KT |
1:100 |
