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. Author manuscript; available in PMC: 2025 Apr 30.
Published in final edited form as: Structure. 2025 Feb 11;33(4):677–690.e5. doi: 10.1016/j.str.2025.01.019

Key Resources Table.

REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
Bacterial and virus strains
ArcticExpress (DE3) cells Agilent Cat#230192
E. coli XL10-Gold Ultracompetent cells Agilent Cat#200315
E. coli BL21-Gold (DE3) cells Agilent Cat#230132
Biological samples
Chemicals, peptides, and recombinant proteins
HEPES Fisher Scientific Cat#BP310-1
NaCl Fisher Scientific Cat# S271-3
Tween 20 Fisher Scientific Cat# BP337-500
10 mM Na acetate pH 4.5 Bio Rad Cat#1762121
Magnesium chloride hexahydrate Sigma-Aldrich Cat#M9272-500G
Imidazole, ReagentPlus®, 99% Sigma-Aldrich Cat#I202-500G
PBS, 10x, pH 7.4 Thermo-Scientific Cat#J62036-K3
Tris-base Fisher Scientific Cat#BP152-1
Sucrose Fisher Scientific Cat#BP220-212
EDTA Fisher Scientific Cat# BP120-1
Glycine Sigma Cat#G7126-5kg
BamHI-HF NEB Cat#R3136S
XhoI NEB Cat#R0146S
T4 DNA ligase NEB Cat#M0202S
SYPRO® Orange Protein Gel Stain Millipore-Sigma Cat# S5692-500UL
LaG16 Fridy et al.4 N/A
LaG19 Fridy et al.4 N/A
LaG21 Fridy et al.4 N/A
LaG24 Fridy et al.4 N/A
LaG35 Fridy et al.4 N/A
LaG41 Fridy et al.4 N/A
LaG43 Fridy et al.4 N/A
LaG43N78R This paper N/A
LaG43K80R This paper N/A
LaG43N81R This paper N/A
LaG19S102R This paper N/A
LaG19Q103V This paper N/A
LaG19Q103I This paper N/A
LaG21L69F This paper N/A
LaG16E91K This paper N/A
LaG24Q68K This paper N/A
LaG24H85Q This paper N/A
S1-8 Mast et al. N/A
S1-8L70F This paper N/A
S1-28 Mast et al.2 N/A
S1-28L70F This paper N/A
S1-36 Mast et al.2 N/A
S1-36L70F This paper N/A
S1-40 Mast et al.2 N/A
S1-40S89K This paper N/A
S1-RBD-14 Mast et al.2 N/A
S1-RBD-14E89K This paper N/A
S1-RBD-42 Mast et al.2 N/A
S1-RBD-42E88K This paper N/A
S1-14 Mast et al.2 N/A
S1-14Q66K This paper N/A
S1-RBD-31 Mast et al.2 N/A
S1-RBD-31Q67K This paper N/A
Deposited data
LaG16:GFP complex This paper PDB ID: 8SFS
LaG43:GFP complex This paper PDB ID: 8SLC
LaG24:GFP complex This paper PDB ID: 8G0I
LaG19:GFP complex This paper PDB ID: 8SFV
LaG21:GFP complex This paper PDB ID: 8SFX
LaG41:GFP complex This paper PDB ID: 8SG3
LaG35:GFP complex This paper PDB ID: 8SFZ
Critical commercial assays
ProteOn Amine Coupling Kit Bio-Rad Cat#1762410
Amine Coupling Kit Cytiva Cat#BR100050
MCSG-1 crystallization screen Molecular dimensions Cat# MCSG-1
MCSG-2 crystallization screen Molecular dimensions Cat# MCSG-2
MCSG-4 crystallization screen Molecular dimensions Cat# MCSG-4
Experimental models: Cell lines
Experimental models: Organisms/strains
Oligonucleotides
Recombinant DNA
pET21-pelB Fridy et al.4 N/A
Software and algorithms
UCSF ChimeraX Meng et al. 42 https://www.rbvi.ucsf.edu/chimerax/download.html
HKL3000 Otwinowski et al.43 https://www.hkl-xray.com/hkl-3000
COOT Emsley and Cowtan46 https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/
PHENIX Liebschner et al.47 https://phenix-online.org/
CCP4 software suit (incudes Mosflm, Phaser, Scala, Procheck) Winn et al.44 https://www.ccp4.ac.uk
PyMOL Schrodinger, LLC https://pymol.org/
ProteOn Manager software Bio-Rad N/A
Biacore Insight Evaluation software Cytiva Cat#29310602
openMM molecular simulation toolkit Eastman et al.49 https://openmm.org/
pyRosetta Mandell et al.52 https://www.pyrosetta.org/
mdtraj Python package McGibbon et al.53 https://www.mdtraj.org/1.9.8.dev0/index.html
Other
ProteOn GLC Sensor Chip Bio-Rad Cat#176-5011
Series S Sensor Chip CM5 Cytiva Cat#BR100530
Hard-shell PCR plates, 96 well, thin-wall Bio-Rad Cat#HSP9661
96-well INTELLI-plates Art Robbins Instruments, Cat#MAR-102-0011-00
TALON® Metal Affinity Resin Takara Bio Cat# 635504
Superdex 75 10/300 GL size-exclusion column Cytiva Cat#17517401
Microseal 'B' Adhesive Seals MSB1001BEDU Bio-Rad Cat# MSB1001