| Materials needed |
Upper endoscopy 6F Liguory catheter
(COOK Medical, Bloomington, Ind, USA)
|
|
|
Sterile gloves / Sterile cap / 5-mL sterile
syringe. |
| Initial test preparation |
Prepare the catheter assembly and aspiration
kit using sterile gloves. |
| Introduction of endoscope |
A sterilized upper endoscope, flushed with
sterile water before intubation, is passed into the small
bowel with minimal air insufflation. |
| Catheter insertion |
Endoscopist staff changes to sterile gloves
during specimen collection. Then, he passes the Liguory
catheter through the biopsy channel of the scope, using a
short overtube to prevent valve contamination. |
| Aspiration |
1. The assistant managing the syringe typically
sits for gravity-assisted suction, gently aspirating fluid
by repeated suctions with a 5 mL syringe connected to a
3-way stopcock. |
|
|
2. Between 3 to 5 minutes, 2-5 mL of
bile-stained small bowel juice is successfully aspirated.
(If aspirate collection is delayed, massaging the liver area
can facilitate bile flow into the intestinal lumen). |
|
|
3. The syringe is capped with a sterile cap,
immediately placed in a biohazard bag, and sent to the
microbiology laboratory for aerobic and anaerobic
cultures. |
