Table 2. Binding Models Best-Fit to the Binding Curves Obtained by Microbore FGCa.
| albumin | ligand | model | binding parameters |
|---|---|---|---|
| HSA | R-Wrf | (2, Kd1, Kd2, a) | Kd1 = 8.67 ± 0.91 μM Kd2 = 38.9 ± 5.2 μM a = 0.00305 ± 0.00021 μM–1 |
| HSA | S-Wrf | (2, Kd1, Kd2, a) | Kd1 = 6.36 ± 0.44 μM Kd2 = 88.7 ± 8.7 μM a = 0.00357 ± 0.00021 μM–1 |
| BSA | S-Wrf | (2, Kd1, Kd2) | Kd1 = 258 ± 3 μM Kd2 = 690 ± 3 μM |
| pig | S-Wrf | (2, Kd, a) | Kd = 11.9 ± 0.5 μM a = 0.00351 ± 0.00010 μM–1 |
| rabbit | S-Wrf | (2, Kd1, Kd2, a) | Kd1 = 37.7 ± 8.3 μM Kd2 = 277 ± 137 μM a = 0.00156 ± 0.00038 μM–1 |
| rat | S-Wrf | (2, Kd1, Kd2, a) | Kd1 = 1.34 ± 0.05 μM Kd2 = 128 ± 93 μM a = 0.00834 ± 0.00395 μM–1 |
| BSA | MO | (3, Kd, a) | Kd = 447 ± 18 μM a = 0.000938 ± 0.000143 μM–1 |
| BSA | Dabs-Gly | (2, Kd) | Kd = 63.3 ± 4.7 μM |
| BSA | Dabs-l-Ala | (3, Kd1, Kd2, Kd3) | Kd1 = 157 ± 16 μM Kd2 = 157 ± 16 μM Kd3 = 256 ± 45 μM |
| BSA | Dabs-d-Ala | (3, Kd1, Kd2, Kd3) | Kd1 = 92 ± 12 μM Kd2 = 92 ± 12 μM Kd3 = 393 ± 142 μM |
a
All the binding data were obtained at 25 °C and in 50 mM HEPES buffer (pH 7.5, ionic strength = 0.15) by microbore FGC. MO, methyl orange; Wrf, warfarin.