Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Peptide, recombinant protein (Homo sapiens) | Pannexin1 | UniProt | Q96RD7 | Coding DNA was synthesized and subcloned into multiple expression vectors |
| Peptide, recombinant protein (Homo sapiens) | Pannexin2 | UniProt | Q96RD6 | Coding DNA was synthesized and subcloned into multiple expression vectors |
| Peptide, recombinant protein (Homo sapiens) | Pannexin3 | UniProt | Q96QZ0 | Coding DNA was synthesized and subcloned into multiple expression vectors |
| Peptide, recombinant protein (Xenopus tropicalis) | Pannexin1 | UniProt | B3DLA5 | Coding DNA was synthesized and subcloned into multiple expression vectors |
| Peptide, recombinant protein (Homo sapiens) | Connexin-43 | UniProt | P17302 | Coding DNA was synthesized and subcloned into multiple expression vectors |
| Peptide, recombinant protein (Homo sapiens) | LRRC8A | UniProt | Q8IWT6 | Coding DNA was synthesized and subcloned into multiple expression vectors |
| Peptide, recombinant protein (Caenorhabditis elegans) | Innexin-6 | UniProt | Q9U3N4 | Coding DNA was synthesized and subcloned into multiple expression vectors |
| Recombinant DNA reagent | pIE2 (plasmid) | This paper | Kawate lab plasmid, mammalian expression vector with IRES-GFP | |
| Recombinant DNA reagent | pCNG-FB7 (plasmid) | This paper | Kawate lab plasmid, insect cell expression vector | |
| Recombinant DNA reagent | pEZT-BM (plasmid) | Addgene | 74099 | |
| Cell line (Spodoptera frugiperda) | Sf9 | ThermoFisher | 12659017 | RRID:CVCL_0549 |
| Cell line (Trichoplusia ni) | High Five | ThermoFisher | B85502 | RRID:CVCL_C190 |
| Cell line (Homo-sapiens) | HEK293 | ATCC | CRL-1573 | RRID:CVCL_0045 |
| Cell line (Homo-sapiens) | HEK293T | ATCC | CRL-3216 | RRID:CVCL_0063 |
| Cell line (Homo-sapiens) | HEK293S GnTI- | ATCC | CRL-3022 | RRID:CVCL_A785 |
| Cell line (Homo-sapiens) | THP-1 | ATCC | TIB-202 | RRID:CVCL_0006 |
| Transfected construct (Homo-sapiens) | PANX1- shRNA #1 | BROAD Institute Genetic Perturbation Platform | TRCN0000155348 | Lentiviral construct to transfect and express the shRNA |
| Transfected construct (Homo-sapiens) | PANX1- shRNA #2 | BROAD Institute Genetic Perturbation Platform | TRCN0000154636 | Lentiviral construct to transfect and express the shRNA |
| Antibody | anti-FLAG M2 (Mouse monoclonal) | Millipore Sigma | F1804 | WB (1:2000) RRID:AB_262044 |
| Antibody | anti-actin (Mouse monoclonal) | Millipore Sigma | A4700 | WB (1:2000) RRID:AB_476730 |
| Antibody | anti-Panx1 (Rabbit monoclonal) | Cell Signaling Technologies | 91137 | WB (1:1000) RRID:AB_2800167 |
| Antibody | anti-cleaved IL-1β (Rabbit monoclonal) | Cell Signaling Technologies | 83186 | WB (1:1000) RRID:AB_2800010 |
| Antibody | anti-cleaved pro-IL-1β (Mouse monoclonal) | Cell Signaling Technologies | 12242 | WB (1:1000) RRID:AB_2715503 |
| Antibody | anti-NLRP3 (Rabbit monoclonal) | Cell Signaling Technologies | 15101 | WB (1:1000) RRID:AB_2722591 |
| Antibody | anti-Caspase 1 (Mouse monoclonal) | AdipoGen | AG-20B-0048 | WB (1:1000) RRID:AB_2490257 |
| Antibody | anti-rabbit IgG HRP-linked | Cell Signaling Technologies | 7074 | WB (1:5000) RRID:AB_2099233 |
| Antibody | anti-mouse IgG HRP-linked | Cell Signaling Technologies | 7076 | WB (1:5000) RRID:AB_330924 |