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Measurement of the temperature of the kidneys during freezing using different cryofixation methods. (a) Three temperature monitoring sites in the kidney. (b) – (h) Average measured temperature curves of EV-AF (b), EV-LN2 (c), EV-Iso (d), IV- LN2 (e), IV-Iso (f), IVI-LN2 (g), and IVI-Iso (h) cryofixation methods. (N = 9 for EV-AF, N = 8 for EV-LN2, N = 7 for IV-LN2 and EV-Iso, N = 6 for IV-Iso, IVI- LN2 and IVI-Iso).
