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Statistics of freezing efficiencies from 20 °C to −100 °C using different cryofixation methods. (a) Cooling time. (b) Normalized reaction amount. (* P < 0.05, ** P < 0.01, *** P < 0.001, and N = 9 for EV-AF, N = 8 for EV-LN2, N = 7 for IV-LN2 and EV-Iso, N = 6 for IV-Iso, IVI- LN2 and IVI-Iso).
