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Comparison of renal metabolic states by using different cryofixation methods. (a) NADH, FAD, and RR images of typical kidney cross-sections by different cryofixation methods. The red arrows indicate kidney cracking, (b) RR statistical analysis. (MeanRR: mean value of RR; R: range; SD: standard deviation; RSD: relative standard deviation. * P < 0.05, ** P < 0.01, *** P < 0.001, and N = 5).
