Figure 6. Validation of epitopes predicted by 3D10 binding criteria.
(A) ELISA reactivity of 3D10 against a panel of recombinant proteins. (B) Comparison of experimental and predicted binding of 3D10 to various Plasmodium antigens. 3D10 binding to peptide arrays was defined by any peptide in the array that could be classified as 3D10 reactive, as characterized in Figure S1. Green shading indicates binding, or satisfaction of criteria and gray indicates no binding or failure to satisfy criteria. (C) Reactivity of 3D10 to DBL proteins treated with DTT to disrupt disulfide bonds. (D-E) Peptide array reactivity for peptides spanning the ID1-ID2 region of VAR2CSA (FCR3) (D) and PcDBP (E).
