Reagents and tools table
| Reagent/resource | Reference or source | Identifier or catalog number |
|---|---|---|
| Experimental models | ||
| E. coli BL21 FtsH-FLAG (C-terminal) | This work following: Reisch CR and Prather KLJ (2017) | Strain AG1_FR1_B1_A1 |
| BL21 FtsH-FLAG (C-terminal) ΔhflK/C | This work following: Reisch CR and Prather KLJ (2017) | Strain AG2_Fr1_B1_A2 |
| Recombinant DNA | ||
| BL21_C-FLAG construct | Integrated DNA Technologies, Inc. | Sequence provided as source data file BL21_C-flag.dna |
| pKDsgRNA-p15a | Addgene | Plasmid #62656 |
| pCas9-CR4 | Addgene | Plasmid #62655 |
| GTGAAAGCACTGATTGAGCGTAACTATAATCGTGCGCGTCAGCTTCTGACCGACAATATGGATATTCTGCATGCGATGAAAGATGCTCTCATGAAATATGAGACTATCGACGCACCGCAGATTGATGACCTGATGGCACGTCGCGATGTACGTCCGCCAGCGGGCTGGGAAGAACCAGGCGCTTCTAACAATTCTGGCGACAATGGTAGTCCAAAGGCTCCTCGTCCGGTTGATGAACCGCGTACGCCGAACCCGGGTAACACGATGTCAGAGCAGTTAGGCGACAAGAGCGGGTCAGGAGACTATAAGGACGACGACGACAAGTAA | Integrated DNA Technologies, Inc. | Geneblock sequence containing FtsH Flag Tag (shown underline) and protospacer (shown in bold) |
| Antibodies | ||
| NA | NA | NA |
| Oligonucleotides and other sequence-based reagents | ||
| Recombineering oligos | Integrated DNA Technologies, Inc. | NA |
| Chemicals, enzymes, and other reagents | ||
| Pyruvate Kinase/Lactic Dehydrogenase enzymes from rabbit muscle | Sigma Aldrich, Inc. | P0294 |
| NADH, grade II | Roche, Inc. | CAS# 606688 |
| Phosphoenolpyruvate | Roche, Inc. | 10108294001 |
| Carboxy-DIBMA | Dr. Sandro Keller’s lab University of Graz | Described in this work |
| Graphene-supported grids | Grassetti et al, 2023 | 200-mesh Quantifoil 2/1 copper grids, pre-treated with UV/ozone for 7 min using a Bioforce UV/ozone cleaner |
| 1-myristoyl-2-{6-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]hexanoyl}-sn-glycero-3-phosphocholine | Avanti Inc. | 810122 |
| POPC | Avanti Inc. | 850457 |
| POPE | Avanti Inc. | 850757 |
| NBD-PE | Avanti Inc. | 810153C |
| DDM | Anatrace Inc. | D310 |
| GDN | Anatrace Inc. | GDN101 |
| Bio-Beads SM-2 Adsorbents #1523920 | Bio-Rad Inc. | #1523920 |
| S-Trap Micro Columns | Protifi inc. | C02-micro-80 |
| Software | ||
| CryoSPARC | Punjani et al, 2017 | V3.0; v4.0 |
| ChimeraX | Pettersen et al, 2021 | V1.2 |
| Coot | Casanal et al, 2020 | V0.9.4 |
| Phenix | Liebschner et al, 2019 | V1.14 |
| cryoDRGN | Zhong et al, 2021 | V0.3.5 |
| Warp | Tegunov and Cramer, 2019 | V1.0.9 |
| IMOD | Mastronarde and Held, 2017 | V4.11.12 |
| RELION | Zivanov et al, 2018 | V3.1.3 |
| M | Tegunov et al, 2021 | V1.0.9 |
| tomoDRGN | Powell and Davis, 2024 | V0.2.2 |
| Spectronaut | Biognosys AG | V15 |
| Other | ||
| Titan Krios G3i – Gatan BioQuantum K3 | Thermo Fisher and Gatan | n/a |
| Q-exactive HF-X | Thermo Fisher | n/a |