Reagents and tools table
| Reagent/resource | Reference or source | Identifier or catalog number |
|---|---|---|
| Experimental models | ||
| Bacterial strains | This study | Table EV1 |
| Bacteriophage strains | This study | Table EV1 |
| Recombinant DNA | ||
| pRE118_Sent∆waaL | This paper | N/A |
| pRE118_Sent∆waaJ | This paper | N/A |
| pRE118_Sent∆waaO | This paper | N/A |
| pRE118_Sent∆waaG | This paper | N/A |
| pRE118_Sent∆waaK | This paper | N/A |
| pRE118_Sent∆waaB | This paper | N/A |
| pRE118_Sent∆waaQ | This paper | N/A |
| pRE118_Sent∆waaY | This paper | N/A |
| pRE118_Sent∆wzzB | This paper | N/A |
| pRE118_Sent∆fepE | This paper | N/A |
| pPSV39_SentWzzB | This paper | N/A |
| pPSV39_SentFepE | This paper | N/A |
| pRE118_Ecoli∆waaO | This paper | N/A |
| pRE118_Crod∆waaO | This paper | N/A |
| pETDuet-1-His6_P22TSP | This paper | N/A |
| pETDuet-1-His6_P22TSP D392N | This paper | N/A |
| pGRG37 | McKenzie et al, 2006 | N/A |
| Oligonucleotides and other sequence-based reagents | ||
| PCR primers | Mission Biotech | Table EV2 |
| Chemicals, enzymes, and other reagents | ||
| Chloramphenicol | Sigma-Aldrich | 56-75-7 |
| Kanamycin | Sigma-Aldrich | 25389-94-0 |
| Streptomycin | Sigma-Aldrich | 3810-74-0 |
| Nalidixic acid | Sigma-Aldrich | 389-08-2 |
| Gentamycin | Sigma-Aldrich | 1405-41-0 |
| Difco LB broth | BD Biosciences | 244610 |
| BHI medium | Sigma-Aldrich | 75917 |
| Sucrose | Sigma-Aldrich | 57-70-1 |
| Isopropyl-β-D-thiogalactopyranoside (IPTG) | Sigma-Aldrich | I1284-5ML |
| Gibson Assembly® Master Mix | New England Biolabs | E2611L |
| XbaI restriction enzyme | New England Biolabs | R0145L |
| SacI restriction enzyme | New England Biolabs | R3156S |
| BamHI restriction enzyme | New England Biolabs | R3136S |
| NotI restriction enzyme | New England Biolabs | R3189S |
| EZ-Tn5<KAN-2>Tnp Transposome Kit | Epicentre BioTechnologies | TSM99K2 |
| DNase I | Thermo Scientific | EN0521 |
| Mitomycin C | Sigma-Aldrich | 50-07-7 |
| Tris-base | Sigma-Aldrich | PT-CHTS-1KG |
| Sodium dodecyl sulfate (SDS) | Sigma-Aldrich | 151-21-3 |
| β-mercaptoethanol | Sigma-Aldrich | 60-24-2 |
| Bromophenol Blue | Sigma-Aldrich | 115-39-9 |
| Proteinase K | Invitrogen | 25530049 |
| Uranyl acetate | Sigma-Aldrich | 20816-12-0 |
| Imidazole | Sigma-Aldrich | 288-32-4 |
| Dithiothreitol (DTT) | Roche | 3483-12-3 |
| Protease inhibitor cocktail | Roche | 04693132001 |
| 1-N-phenylnaphthylamine (NPN) | Sigma-Aldrich | 90-30-2 |
| Vancomycin (VAN) | Sigma-Aldrich | 1404-93-9 |
| HEPES | Sigma-Aldrich | 7365-45-9 |
| EDTA | Sigma-Aldrich | 10378-23-1 |
| Software | ||
| Fiji | ImageJ | |
| Nanoscope Analysis software | Bruker AXS | |
| Geneious Prime | Dotmatics | |
| GraphPad Prism | Dotmatics | |
| Adobe Illustrator | Adobe | |
| Other | ||
| Nitrocellulose filter | Cytiva | |
| Pro-Q Emerald 300 LPS extraction Gel Stain kit | Invitrogen | |
| DNeasy Blood & Tissue Kit | Qiagen | |
| QIAprep Spin Miniprep Kit | Qiagen | |
| Minisart 0.2 µm filters | Sartorius | |
| Minisart 0.45 µm filters | Sartorius | |
| SPRIselect Bead-Based Reagent | Beckman Coulter | |
| Terminal deoxynucleotidyl transferase (TdT) | New England Biolabs | |
| Infinite F200 series plate reader | Tecan | |
| SpectraMax iD5 microplate reader | Molecular Devices | |
| Carbon-coated formvar film | Ted Pella Inc. | |
| Talos 120 C transmission electron microscope | Thermo | |
| Stratalinker UV Crosslinker 1800 | Stratagene | |
| Ni-NTA agarose | Qiagen | |
| Multimode NanoScope V system | Bruker AXS | |
| Silicon cantilever | FMAuD, NanoSensor | |