Reagents and tools table
Reagent/Resource | Reference or Source | Identifier or Catalog Number |
---|---|---|
Experimental models | ||
Rice stripe virus Jiangsu isolate (JSHA) | Zhao et al, 2018 | N/A |
SBPH populations | This paper | N/A |
O. sativa subsp. japonica wuyujing3 | This paper | N/A |
O. sativa subsp. japonica cv. Nipponbare Wild-type | This paper | N/A |
O. sativa subsp. japonica cv. Nipponbare OE263a | This paper | N/A |
O. sativa subsp. japonica cv. Nipponbare GATA19KO | This paper | N/A |
Rice protoplasts | This paper | N/A |
Nicotiana benthamiana | This paper | N/A |
GV3101 Agrobacterium Competent Cells | Zoman | Cat# ZK295 |
Drosophila S2 cells | Thermo Scientific | Cat# R69007 |
Recombinant DNA | ||
psiCHECK2 | Promega | Cat# C8021 |
pciCHECK2-CathBL-5′UTR | This paper | N/A |
pciCHECK2-SPS5-5′UTR | This paper | N/A |
pciCHECK2-NTFYbL-3′UTR | This paper | N/A |
pAC5.1B | Invitrogen | Cat# V411020 |
pAC5.1B-CathBL | This paper | N/A |
pBI221 | MiaoLing | Cat# P6700 |
pBI221-EGFP | HonorGene | Cat# VZC0336 |
pBI221-EGFP-targe | This paper | N/A |
pBI-OE263a | This paper | N/A |
pBI-Ex-In-Ex | This paper | N/A |
pBI-Ex-InMT-Ex | This paper | N/A |
Antibodies | ||
Anti-GATA19 polyclonal antibody | This paper | N/A |
Anti-RSV NP monoclonal antibody | Zhao et al, 2016b | N/A |
Anti-Ago1 monoclonal antibody | Zhao et al, 2019a | N/A |
Anti-GAPDH polyclonal antibody | Wang et al, 2013 | N/A |
Anti-Histone H3 Mouse Monoclonal Antibody | EASYBIO | Cat# BE3021 |
Anti-plant-Actin Mouse Monoclonal Antibody | EASYBIO | Cat# BE0028 |
Anti-V5-Tag Mouse Monoclonal Antibody | EASYBIO | Cat# BE2032 |
Anti-His-Tag Mouse Monoclonal Antibody | EASYBIO | Cat# BE2019 |
Goat Anti-Mouse IgG (H&L)-HRP | EASYBIO | Cat# BE0102 |
Goat Anti-Rabbit IgG (H&L)-HRP | EASYBIO | Cat# BE0101 |
Goat anti-Mouse IgG (Fc specific, Heavy Chain), HRP | EASYBIO | Cat# BE0104 |
Goat anti-Rabbit IgG (Fc specific, Heavy Chain), HRP | EASYBIO | Cat# BE0106 |
Ago1/Anti-Argonaute 1 Antibody | PhytoAB | Cat# PHY1381S |
Ago4/ Anti-Argonaute 4 Antibody | PhytoAB | Cat# PHY0202S |
Recombinant Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody | Abcam | Cat# ab205606 |
Goat Anti-Mouse IgG H&L | Abcam | Cat# ab6708 |
Streptavidin, HRP labeled | Biodragon | Cat# BF06185X |
Alexa Fluor 488 conjugated secondary antibody | Invitrogen | Cat# A-11008 |
Oligonucleotides and other sequence-based reagents | ||
Primers and probes | This paper | Appendix Table S1 |
Chemicals, Enzymes and other reagents | ||
Caspase inhibitor Z-VAD-FMK | Beyotime | Cat# C1202-0.02ml |
CA-074 methyl ester | ChemeGen | Cat# 147859-80-1 |
Chloroform | Sigma-Aldrich | Cat# C2432-500M |
Dimethyl sulfoxide (DMSO) | Sigma-Aldrich | Cat# D2650 |
Protease Inhibitor Cocktail | MilliporeSigma | Cat# P8849 |
M-MLV Reverse Transcriptase | Promega | Cat# PAM1701 |
Oligo(dT)15 Primer | Promega | Cat# PAC1101 |
Random Pimers | Promega | Cat# PAC1181 |
Ampicillin | Sangon | Cat# B541011 |
BrightStar™-Plus Positively Charged Nylon Membrane | Invitrogen | Cat# AM10102 |
Glycogen (5 mg mL−1) | Invitrogen | Cat# AM9510 |
Lipofectamine™ 3000 Transfection Reagent | Invitrogen | Cat# 11668019 |
North2SouthTM Hybridization Stringency Wash Buffer (2×) | Thermo Scientific | Cat# 37555 |
Nucleic Acid Detection Blocking Buffer | Thermo Scientific | Cat# 89880A |
Substrate Equilibration Buffer | Thermo Scientific | Cat# 89880C |
TRIzol™ Reagent | Invitrogen | Cat# 15596026 |
UltraPure™ DNase/RNase-Free Distilled Water | Invitrogen | Cat# 10977015 |
UltraPure™ SSC, 20X | Invitrogen | Cat# 15557036 |
2×RNA Loading Dye | NEB | Cat# B0363S |
T4 DNA Ligase | NEB | Cat# M0202S |
T4 ligase reaction buffer | NEB | Cat# B0216S |
dNTPs (2.5 mM each) | Tiangen | Cat# CD117-02 |
10×TBE buffer | Solarbio | Cat# T1051 |
Acid phenol chloroform | Merck | Cat# 77619 |
Sodium periodate, AR, 99.5% | Macklin | Cat# S817518 |
Borate Buffer | Macklin | Cat# B885515 |
Super Hybridization Solution | Zeyebio | Cat# ZY7352PD |
Nucleic Acid Pre-Hybridization Solution | Zeyebio | Cat# ZY601922B |
FDA (10 mg ml−1) | Coolaber | Cat# PPT5351 |
4% Paraformaldehyde Fix Solution (PFA) | BBI Life Science | Cat# E672002-0100 |
VAD-FMK | Abcam | Cat# ab270770 |
Caspase 3 Activity Assay Kit | Beyotime | Cat# C1116 |
Caspase 8 Activity Assay Kit | Beyotime | Cat# C1152 |
One-Step TUNEL Apoptosis Assay Kit (Red Fluorescence) | Beyotime | Cat# C1089 |
Cathepsin B Assay Kit (Magic Red) | Abcam | Cat# Ab270772 |
Dual-Glo® Luciferase Assay System | Promega | Cat# E2920 |
Dynabeads Protein G | Invitrogen | Cat# 10003D |
T7 RiboMAX™ Express RNAi System | Promega | Cat# P1700 |
KOD-Plus-Mutagenesis Kit | Toyobo | Cat# SMK-101 |
miRcute Plus miRNA First-Strand cDNA Kit | Tiangen | Cat# 4992909 |
miRcute Plus miRNA qPCR Kit (SYBR Green) | Tiangen | Cat# 4992779 |
Premium One Cell-Free Protein Expression Kit | Tiangen | Cat# CFS-EDX-ONE |
In-Fusion HD Cloning kit | Takara | Cat# 638943 |
FirstChoice™ RLM-RACE | Invitrogen | Cat# AM1700M |
pEASY®-Blunt Zero Cloning Kit | TRANS | Cat# CB501-01 |
Plant nucleus and cytoplasm extraction kit | Bestbio | Cat# BB-31124 |
Rice Protoplast Preparation and Transformation Kit | Coolaber | Cat# PPT111-5T |
SuperSignal West Femto | Thermo Scientific | Cat# 34094 |
Superscript III First-Strand Synthesis System | Thermo Scientific | Cat# 18080051 |
Magna RIP RNA-binding protein immunoprecipitation kit | Merck | Cat# 17-700 |
Software | ||
GraphPad Prism 9.0 | https://www.graphpad.com/ | N/A |
SPSS statistics 19.0 | https://spss.software.informer.com/download/ | N/A |
ImageJ | https://imagej.net/ij/ | N/A |
LightCycler480 | https://lightcycler-961.software.informer.com/download/ | N/A |
miRanda | http://www.microrna.org/microrna | N/A |
RNAhybrid | https://bibiserv.cebitec.un-ibielefeld.de/rnahybrid | N/A |
psRNATarget | http://plantgrn.noble.org/psRNATarget/ | N/A |
Other | ||
RNA-seq reads (Full-length transcriptome of SBPH) | Tong et al, 2022 | PRJCA005421 |
RNA-seq reads (Gut transcriptom of SBPH) | Zhao et al, 2016a | SRP048969 |
Oryza sativa (rice) genomic DNA data | http://rice.uga.edu/analyses_search_blast.shtml | N/A |