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. 2025 Mar 5;44(9):2446–2472. doi: 10.1038/s44318-025-00405-4

Reagents and tools table

Reagent/Resource Reference or Source Identifier or Catalog Number
Experimental models
Rice stripe virus Jiangsu isolate (JSHA) Zhao et al, 2018 N/A
SBPH populations This paper N/A
O. sativa subsp. japonica wuyujing3 This paper N/A
O. sativa subsp. japonica cv. Nipponbare Wild-type This paper N/A
O. sativa subsp. japonica cv. Nipponbare OE263a This paper N/A
O. sativa subsp. japonica cv. Nipponbare GATA19KO This paper N/A
Rice protoplasts This paper N/A
Nicotiana benthamiana This paper N/A
GV3101 Agrobacterium Competent Cells Zoman Cat# ZK295
Drosophila S2 cells Thermo Scientific Cat# R69007
Recombinant DNA
psiCHECK2 Promega Cat# C8021
pciCHECK2-CathBL-5′UTR This paper N/A
pciCHECK2-SPS5-5′UTR This paper N/A
pciCHECK2-NTFYbL-3′UTR This paper N/A
pAC5.1B Invitrogen Cat# V411020
pAC5.1B-CathBL This paper N/A
pBI221 MiaoLing Cat# P6700
pBI221-EGFP HonorGene Cat# VZC0336
pBI221-EGFP-targe This paper N/A
pBI-OE263a This paper N/A
pBI-Ex-In-Ex This paper N/A
pBI-Ex-InMT-Ex This paper N/A
Antibodies
Anti-GATA19 polyclonal antibody This paper N/A
Anti-RSV NP monoclonal antibody Zhao et al, 2016b N/A
Anti-Ago1 monoclonal antibody Zhao et al, 2019a N/A
Anti-GAPDH polyclonal antibody Wang et al, 2013 N/A
Anti-Histone H3 Mouse Monoclonal Antibody EASYBIO Cat# BE3021
Anti-plant-Actin Mouse Monoclonal Antibody EASYBIO Cat# BE0028
Anti-V5-Tag Mouse Monoclonal Antibody EASYBIO Cat# BE2032
Anti-His-Tag Mouse Monoclonal Antibody EASYBIO Cat# BE2019
Goat Anti-Mouse IgG (H&L)-HRP EASYBIO Cat# BE0102
Goat Anti-Rabbit IgG (H&L)-HRP EASYBIO Cat# BE0101
Goat anti-Mouse IgG (Fc specific, Heavy Chain), HRP EASYBIO Cat# BE0104
Goat anti-Rabbit IgG (Fc specific, Heavy Chain), HRP EASYBIO Cat# BE0106
Ago1/Anti-Argonaute 1 Antibody PhytoAB Cat# PHY1381S
Ago4/ Anti-Argonaute 4 Antibody PhytoAB Cat# PHY0202S
Recombinant Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody Abcam Cat# ab205606
Goat Anti-Mouse IgG H&L Abcam Cat# ab6708
Streptavidin, HRP labeled Biodragon Cat# BF06185X
Alexa Fluor 488 conjugated secondary antibody Invitrogen Cat# A-11008
Oligonucleotides and other sequence-based reagents
Primers and probes This paper Appendix Table S1
Chemicals, Enzymes and other reagents
Caspase inhibitor Z-VAD-FMK Beyotime Cat# C1202-0.02ml
CA-074 methyl ester ChemeGen Cat# 147859-80-1
Chloroform Sigma-Aldrich Cat# C2432-500M
Dimethyl sulfoxide (DMSO) Sigma-Aldrich Cat# D2650
Protease Inhibitor Cocktail MilliporeSigma Cat# P8849
M-MLV Reverse Transcriptase Promega Cat# PAM1701
Oligo(dT)15 Primer Promega Cat# PAC1101
Random Pimers Promega Cat# PAC1181
Ampicillin Sangon Cat# B541011
BrightStar™-Plus Positively Charged Nylon Membrane Invitrogen Cat# AM10102
Glycogen (5 mg mL−1) Invitrogen Cat# AM9510
Lipofectamine™ 3000 Transfection Reagent Invitrogen Cat# 11668019
North2SouthTM Hybridization Stringency Wash Buffer (2×) Thermo Scientific Cat# 37555
Nucleic Acid Detection Blocking Buffer Thermo Scientific Cat# 89880A
Substrate Equilibration Buffer Thermo Scientific Cat# 89880C
TRIzol™ Reagent Invitrogen Cat# 15596026
UltraPure™ DNase/RNase-Free Distilled Water Invitrogen Cat# 10977015
UltraPure™ SSC, 20X Invitrogen Cat# 15557036
2×RNA Loading Dye NEB Cat# B0363S
T4 DNA Ligase NEB Cat# M0202S
T4 ligase reaction buffer NEB Cat# B0216S
dNTPs (2.5 mM each) Tiangen Cat# CD117-02
10×TBE buffer Solarbio Cat# T1051
Acid phenol chloroform Merck Cat# 77619
Sodium periodate, AR, 99.5% Macklin Cat# S817518
Borate Buffer Macklin Cat# B885515
Super Hybridization Solution Zeyebio Cat# ZY7352PD
Nucleic Acid Pre-Hybridization Solution Zeyebio Cat# ZY601922B
FDA (10 mg ml−1) Coolaber Cat# PPT5351
4% Paraformaldehyde Fix Solution (PFA) BBI Life Science Cat# E672002-0100
VAD-FMK Abcam Cat# ab270770
Caspase 3 Activity Assay Kit Beyotime Cat# C1116
Caspase 8 Activity Assay Kit Beyotime Cat# C1152
One-Step TUNEL Apoptosis Assay Kit (Red Fluorescence) Beyotime Cat# C1089
Cathepsin B Assay Kit (Magic Red) Abcam Cat# Ab270772
Dual-Glo® Luciferase Assay System Promega Cat# E2920
Dynabeads Protein G Invitrogen Cat# 10003D
T7 RiboMAX™ Express RNAi System Promega Cat# P1700
KOD-Plus-Mutagenesis Kit Toyobo Cat# SMK-101
miRcute Plus miRNA First-Strand cDNA Kit Tiangen Cat# 4992909
miRcute Plus miRNA qPCR Kit (SYBR Green) Tiangen Cat# 4992779
Premium One Cell-Free Protein Expression Kit Tiangen Cat# CFS-EDX-ONE
In-Fusion HD Cloning kit Takara Cat# 638943
FirstChoice™ RLM-RACE Invitrogen Cat# AM1700M
pEASY®-Blunt Zero Cloning Kit TRANS Cat# CB501-01
Plant nucleus and cytoplasm extraction kit Bestbio Cat# BB-31124
Rice Protoplast Preparation and Transformation Kit Coolaber Cat# PPT111-5T
SuperSignal West Femto Thermo Scientific Cat# 34094
Superscript III First-Strand Synthesis System Thermo Scientific Cat# 18080051
Magna RIP RNA-binding protein immunoprecipitation kit Merck Cat# 17-700
Software
GraphPad Prism 9.0 https://www.graphpad.com/ N/A
SPSS statistics 19.0 https://spss.software.informer.com/download/ N/A
ImageJ https://imagej.net/ij/ N/A
LightCycler480 https://lightcycler-961.software.informer.com/download/ N/A
miRanda http://www.microrna.org/microrna N/A
RNAhybrid https://bibiserv.cebitec.un-ibielefeld.de/rnahybrid N/A
psRNATarget http://plantgrn.noble.org/psRNATarget/ N/A
Other
RNA-seq reads (Full-length transcriptome of SBPH) Tong et al, 2022 PRJCA005421
RNA-seq reads (Gut transcriptom of SBPH) Zhao et al, 2016a SRP048969
Oryza sativa (rice) genomic DNA data http://rice.uga.edu/analyses_search_blast.shtml N/A