TABLE 1.

Oligonucleotide primers and LightCycler hybridization probes used in the PCR assay

Oligonucleotide	Sequencea	Target gene	Nucleotide positions	GenBank accession no.	Source
Sa442-F	GTCGGGTACACGATATTCTTCACG	Sa442	12-34	AF033191	This study
Sa442-RS	CTCGTATGACCAGCTTCGGT	Sa442	189-168	AF033191	This study
Sa442-HP-1	TACTGAAATCTCATTACGTTGCATCGGAA-FAM	Sa442	95-123	AF033191	Reischl et al. (19)
Sa442-HP-2	Red 705-ATTGTGTTCTGTATGTAAAAGCCGTCTTG-Ph	Sa442	126-154	AF033191	Reischl et al. (19)
Mec-S	CTAGGTGTGGTGAAGATATACCA	mecA	1596-1619	X52592	This study
Mec-A	TGAGGTGCGTTAATATTGCCA	mecA	1783-1763	X52592	This study
Mec-HP-1	CAGGTTACGGACAAGGTGAAATACTGATT-FAM	mecA	1690-1718	X52592	Reischl et al. (19)
Mec-HP-2	Red 640-ACCCAGTACAGATCCTTTCAATCTATAGCG-Ph	mecA	1720-1739	X52592	Reischl et al. (19)

^aFAM, fluorescein; Red 705, LightCycler Red 705 phosphoramidite; Ph, 3′-phosphate; Red 640, LightCycler Red 640 N-hydroxysuccinimide ester.