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katG 315 PCR-RFLP (A) and MAS-PCR (B) profiles of the M. tuberculosis strains. (A) Lanes: 1, 200-bp PCR fragment used for HapII-RFLP analysis; 2, strain with katG 315 AGC→ACC mutation; 3, strain with katG315 wild-type allele. (B) Lanes: 1, 3, and 4, strains with the katG315 AGC→ACC mutation; 2, 5, and 6, strains with the katG315 wild-type allele; 7, strain with the AGC→ACA mutation (strain W); M, 100-bp DNA ladder (Amersham Pharmacia Biotech).
