Fig. 7. (A) Sketch reporting the illumination conditions for the living cells in the home-made setup. The cells were illuminated from the top (400 μW mm−2) with two different illumination patterns (left), and average variation of Ca2+ peaks under different experimental conditions is shown, after the 20/200 ms stimulation protocol. The statistical significance is reported for p < 0.001 (***), all other differences are not significantly different (right). Reprinted with permission.100 Copyright 2022 The Royal Society of Chemistry. (B) Schematic representation of the biological pathways possibly induced by photostimulation of semiconducting polymer NPs. Reprinted with permission.102 Copyright 2022 The Authors. Advanced Healthcare Materials published by Wiley-VCH GmbH. (C) SEM image of a neuronal soma suspended over two P3HT pillars. (D) Cortical neurons cultured on P3HT micropillars after 3 DIV, where neurons were fixed and fluorescently labeled for β-III-tubulin (green) and Tau-1 (red). (E) Average neurite length for 257 neurons on glass, 225 neurons on flat P3HT, and 229 neurons on pillar P3HT (data were compared using the non-parametric Mann–Whitney U-test with Bonferroni–Holm multiple comparison correction (0.05 significance level). ***p < 0.001, ns—not significant). Reprinted with permission.103 Copyright 2021 American Chemical Society.
