Skip to main content
PMC home page
Genetics logoLink to Genetics
. 1993 Dec;135(4):1063–1076. doi: 10.1093/genetics/135.4.1063

Hobo Enhancer Trapping Mutagenesis in Drosophila Reveals an Insertion Specificity Different from P Elements

D Smith 1, J Wohlgemuth 1, B R Calvi 1, I Franklin 1, W M Gelbart 1
PMCID: PMC1205738  PMID: 8307323

Abstract

P element enhancer trapping has become an indispensable tool in the analysis of the Drosophila melanogaster genome. However, there is great variation in the mutability of loci by these elements such that some loci are relatively refractory to insertion. We have developed the hobo transposable element for use in enhancer trapping and we describe the results of a hobo enhancer trap screen. In addition, we present evidence that a hobo enhancer trap element has a pattern of insertion into the genome that is different from the distribution of P elements in the available database. Hence, hobo insertion may facilitate access to genes resistant to P element insertion.

Full Text

The Full Text of this article is available as a PDF (5.9 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Athma P., Grotewold E., Peterson T. Insertional mutagenesis of the maize P gene by intragenic transposition of Ac. Genetics. 1992 May;131(1):199–209. doi: 10.1093/genetics/131.1.199. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bellen H. J., O'Kane C. J., Wilson C., Grossniklaus U., Pearson R. K., Gehring W. J. P-element-mediated enhancer detection: a versatile method to study development in Drosophila. Genes Dev. 1989 Sep;3(9):1288–1300. doi: 10.1101/gad.3.9.1288. [DOI] [PubMed] [Google Scholar]
  3. Berg C. A., Spradling A. C. Studies on the rate and site-specificity of P element transposition. Genetics. 1991 Mar;127(3):515–524. doi: 10.1093/genetics/127.3.515. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bier E., Vaessin H., Shepherd S., Lee K., McCall K., Barbel S., Ackerman L., Carretto R., Uemura T., Grell E. Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. Genes Dev. 1989 Sep;3(9):1273–1287. doi: 10.1101/gad.3.9.1273. [DOI] [PubMed] [Google Scholar]
  5. Blackman R. K., Grimaila R., Koehler M. M., Gelbart W. M. Mobilization of hobo elements residing within the decapentaplegic gene complex: suggestion of a new hybrid dysgenesis system in Drosophila melanogaster. Cell. 1987 May 22;49(4):497–505. doi: 10.1016/0092-8674(87)90452-1. [DOI] [PubMed] [Google Scholar]
  6. Blackman R. K., Sanicola M., Raftery L. A., Gillevet T., Gelbart W. M. An extensive 3' cis-regulatory region directs the imaginal disk expression of decapentaplegic, a member of the TGF-beta family in Drosophila. Development. 1991 Mar;111(3):657–666. doi: 10.1242/dev.111.3.657. [DOI] [PubMed] [Google Scholar]
  7. Cooley L., Berg C., Spradling A. Controlling P element insertional mutagenesis. Trends Genet. 1988 Sep;4(9):254–258. doi: 10.1016/0168-9525(88)90032-7. [DOI] [PubMed] [Google Scholar]
  8. Cooley L., Kelley R., Spradling A. Insertional mutagenesis of the Drosophila genome with single P elements. Science. 1988 Mar 4;239(4844):1121–1128. doi: 10.1126/science.2830671. [DOI] [PubMed] [Google Scholar]
  9. Hiromi Y., Gehring W. J. Regulation and function of the Drosophila segmentation gene fushi tarazu. Cell. 1987 Sep 11;50(6):963–974. doi: 10.1016/0092-8674(87)90523-x. [DOI] [PubMed] [Google Scholar]
  10. Merriam J., Ashburner M., Hartl D. L., Kafatos F. C. Toward cloning and mapping the genome of Drosophila. Science. 1991 Oct 11;254(5029):221–225. doi: 10.1126/science.254.5029.221. [DOI] [PubMed] [Google Scholar]
  11. Montell D. J., Goodman C. S. Drosophila laminin: sequence of B2 subunit and expression of all three subunits during embryogenesis. J Cell Biol. 1989 Nov;109(5):2441–2453. doi: 10.1083/jcb.109.5.2441. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Montell D. J., Goodman C. S. Drosophila substrate adhesion molecule: sequence of laminin B1 chain reveals domains of homology with mouse. Cell. 1988 May 6;53(3):463–473. doi: 10.1016/0092-8674(88)90166-3. [DOI] [PubMed] [Google Scholar]
  13. Moreno M. A., Chen J., Greenblatt I., Dellaporta S. L. Reconstitutional mutagenesis of the maize P gene by short-range Ac transpositions. Genetics. 1992 Aug;131(4):939–956. doi: 10.1093/genetics/131.4.939. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. O'Kane C. J., Gehring W. J. Detection in situ of genomic regulatory elements in Drosophila. Proc Natl Acad Sci U S A. 1987 Dec;84(24):9123–9127. doi: 10.1073/pnas.84.24.9123. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Perrimon N., Noll E., McCall K., Brand A. Generating lineage-specific markers to study Drosophila development. Dev Genet. 1991;12(3):238–252. doi: 10.1002/dvg.1020120309. [DOI] [PubMed] [Google Scholar]
  16. Pirrotta V., Steller H., Bozzetti M. P. Multiple upstream regulatory elements control the expression of the Drosophila white gene. EMBO J. 1985 Dec 16;4(13A):3501–3508. doi: 10.1002/j.1460-2075.1985.tb04109.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Plasterk R. H. The ins and outs of transposition. Molecular Mechanisms of Transposition and its Control, sponsored by the European Molecular Biology Organization, Roscoff, France, June 24-28, 1990. New Biol. 1990 Sep;2(9):787–792. [PubMed] [Google Scholar]
  18. Ray R. P., Arora K., Nüsslein-Volhard C., Gelbart W. M. The control of cell fate along the dorsal-ventral axis of the Drosophila embryo. Development. 1991 Sep;113(1):35–54. doi: 10.1242/dev.113.1.35. [DOI] [PubMed] [Google Scholar]
  19. Rio D. C. Molecular mechanisms regulating Drosophila P element transposition. Annu Rev Genet. 1990;24:543–578. doi: 10.1146/annurev.ge.24.120190.002551. [DOI] [PubMed] [Google Scholar]
  20. Seifert H. S., Chen E. Y., So M., Heffron F. Shuttle mutagenesis: a method of transposon mutagenesis for Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1986 Feb;83(3):735–739. doi: 10.1073/pnas.83.3.735. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Sheen F., Lim J. K., Simmons M. J. Genetic instability in Drosophila melanogaster mediated by hobo transposable elements. Genetics. 1993 Feb;133(2):315–334. doi: 10.1093/genetics/133.2.315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. St Johnston R. D., Gelbart W. M. Decapentaplegic transcripts are localized along the dorsal-ventral axis of the Drosophila embryo. EMBO J. 1987 Sep;6(9):2785–2791. doi: 10.1002/j.1460-2075.1987.tb02574.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Streck R. D., Macgaffey J. E., Beckendorf S. K. The structure of hobo transposable elements and their insertion sites. EMBO J. 1986 Dec 20;5(13):3615–3623. doi: 10.1002/j.1460-2075.1986.tb04690.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Tower J., Karpen G. H., Craig N., Spradling A. C. Preferential transposition of Drosophila P elements to nearby chromosomal sites. Genetics. 1993 Feb;133(2):347–359. doi: 10.1093/genetics/133.2.347. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Van Schaik N W, Brink R A. Transpositions of Modulator, a Component of the Variegated Pericarp Allele in Maize. Genetics. 1959 Jul;44(4):725–738. doi: 10.1093/genetics/44.4.725. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Wilson C., Pearson R. K., Bellen H. J., O'Kane C. J., Grossniklaus U., Gehring W. J. P-element-mediated enhancer detection: an efficient method for isolating and characterizing developmentally regulated genes in Drosophila. Genes Dev. 1989 Sep;3(9):1301–1313. doi: 10.1101/gad.3.9.1301. [DOI] [PubMed] [Google Scholar]
  27. Zhang P., Spradling A. C. Efficient and dispersed local P element transposition from Drosophila females. Genetics. 1993 Feb;133(2):361–373. doi: 10.1093/genetics/133.2.361. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Genetics are provided here courtesy of Oxford University Press

RESOURCES