TABLE 1.
Agreement between MICs of four antifungals for 49 strains of dermatophytes obtained with the Sensititre YeastOne panel and a microdilution method
| Species (no. of strains tested) | Antifungal agenta | MIC (μg/ml) range
|
No. of isolates for which MICs determined with the Sensititre YeastOne panel differed from MICs determined by microdilution method by the following dilution:
|
% Agreementb | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Sensititre YeastOne panel | Microdilution method | <−2 | −2 | −1 | 0 | +1 | +2 | >+2 | |||
| E. flocossum (8) | AMB | 0.12-0.5 | 0.03-1 | 0 | 0 | 2 | 2 | 1 | 2 | 1 | 87.5 |
| FLC | 0.5-32 | 0.25-64 | 1 | 0 | 2 | 2 | 1 | 1 | 1 | 75 | |
| ITC | ≤ 0.008-0.06 | ≤ 0.01-0.5 | 1 | 0 | 4 | 1 | 2 | 0 | 0 | 87.5 | |
| KTC | ≤ 0.008-0.25 | 0.03-2 | 2 | 1 | 1 | 1 | 1 | 0 | 2 | 50 | |
| M. canis (8) | AMB | 0.25-2 | 0.25-8 | 1 | 0 | 1 | 4 | 0 | 2 | 0 | 87.5 |
| FLC | 8-64 | 0.5-16 | 0 | 0 | 0 | 2 | 1 | 3 | 2 | 75 | |
| ITC | 0.01-0.12 | 0.03-0.12 | 0 | 1 | 3 | 1 | 2 | 1 | 0 | 100 | |
| KTC | 0.25-0.5 | 0.06-0.25 | 0 | 0 | 0 | 0 | 5 | 1 | 2 | 75 | |
| M. gypseum (9) | AMB | 2-8 | 0.12-8 | 0 | 1 | 0 | 0 | 2 | 0 | 6 | 33.3 |
| FLC | 1-64 | 8-32 | 1 | 2 | 1 | 2 | 2 | 1 | 0 | 88.9 | |
| ITC | ≤ 0.008-0.25 | 0.03-0.25 | 0 | 2 | 0 | 4 | 3 | 0 | 0 | 100 | |
| KTC | 0.06-1 | 0.12-2 | 1 | 1 | 3 | 1 | 3 | 0 | 0 | 88.8 | |
| T. mentagrophytes (8) | AMB | 0.12-1 | 0.25-0.5 | 0 | 1 | 0 | 4 | 3 | 0 | 0 | 100 |
| FLC | 4-64 | 8-128 | 4 | 0 | 2 | 1 | 0 | 1 | 0 | 50 | |
| ITC | 0.01-0.12 | 0.03-0.5 | 3 | 2 | 0 | 3 | 0 | 0 | 0 | 62.5 | |
| KTC | 0.03-1 | 0.12-2 | 2 | 1 | 2 | 2 | 0 | 1 | 0 | 75 | |
| T. rubrum (8) | AMB | 0.25-1 | 0.12-1 | 0 | 0 | 2 | 2 | 3 | 1 | 0 | 100 |
| FLC | 0.25-64 | 4-8 | 3 | 1 | 0 | 0 | 1 | 1 | 2 | 37.5 | |
| ITC | ≤ 0.008-0.25 | ≤ 0.01-1 | 1 | 3 | 1 | 1 | 1 | 0 | 1 | 75 | |
| KTC | ≤ 0.008-2 | 0.5-2 | 4 | 0 | 3 | 0 | 0 | 1 | 0 | 50 | |
| T. tonsurans (8) | AMB | 0.12-1 | 0.25-1 | 1 | 1 | 2 | 2 | 2 | 0 | 0 | 87.5 |
| FLC | 1-32 | ≤ 0.12-64 | 0 | 2 | 1 | 1 | 1 | 1 | 2 | 75 | |
| ITC | ≤ 0.008-0.06 | ≤ 0.01-0.03 | 0 | 1 | 2 | 3 | 1 | 1 | 0 | 100 | |
| KTC | ≤ 0.008-0.12 | 0.06-0.5 | 2 | 3 | 3 | 0 | 0 | 0 | 0 | 75 | |
| Overall (49) | AMB | 0.012-8 | 0.03-8 | 2 | 3 | 7 | 14 | 11 | 5 | 7 | 81.6 |
| FLC | 0.25-64 | ≤ 0.12-128 | 9 | 5 | 6 | 8 | 6 | 8 | 7 | 67.3 | |
| ITC | ≤ 0.008-0.25 | ≤ 0.01-1 | 5 | 9 | 10 | 13 | 9 | 2 | 1 | 87.7 | |
| KTC | ≤ 0.008-2 | 0.03-2 | 11 | 6 | 12 | 4 | 9 | 3 | 4 | 69.4 | |
a
AMB, amphotericin B; FLC, fluconazole; ITC, itraconazole; KTC, ketoconazole.
b
Percent agreement defined as discrepancies in MIC endpoints of no more than 2 dilutions.