FIG. 2.

Sensitivity of one-step RT-PCR in the absence (−) and presence (+) of SybrGreen. The dye was lyophilized at the bottom of the capillary. In vitro-transcribed LASV RNA was amplified in a LightCycler as described in Materials and Methods. The PCR products were visualized in ethidium bromide-stained agarose gel. Input RNA copy numbers are indicated above the lanes. The negative control (neg) contained RNA extracted from negative plasma.