TABLE 3.

Amino acid sequences of OspC proteins of southern B. burgdorferi sensu lato and other strains deduced from the nucleotide sequences of ospCs aligned with Clustal W^a

Isolate	Species     specific      sequencesC1V1SVV2
	◂—————▸◂————————————————————————————————-▸◂——————--▸◂—————————-▸◂———————————————————————————————————————————————————————--—▸
SCGT-8a	SAILMTLFLFISCNNSGKDGNSAST-NPADESVKGPNLTEISKKIMDSNTVVLAVKEVEA	59	LLSSIDELATKAIGKKIDANGSLVADATDFNTSLLAGAYAISSLITEKLN---KLKNSEG	116	LKEKIAEAKKCSDDFTKKLKDSHQELGVANGAATADNAKKAILKTNATKDKGAEELEKLF	176	KAVESLSKAAQDMLTNSVKELTSPVVGKSPKX	208
SCGT-10	SAILMTLFLFISCNNSGKDGNSAST-NPADESAKGPNLTEISKKITDSNTVVLAVKEVET	59	LLASIDELATKAIGKKIDANGSLVADATDFNTSLLAGAYAISSLITEKLN---KLKNSEG	116	LKEKIAEAKKCSDDFTKKLKDSHQELGVANGAATADNAKKAILKTNATKDKGAEELEKLF	176	KAVESLSKAAQDMLTNSVKELTSPVVGKSPKX	208
DN127	SAILMTLFLFISCNNSGKDGNSAST-NPADESAKGPNLTEISKKITDSNAIVLAVKEVET	59	LLLSIDELA-KAIGKKINNNG--LDVLQNFNASLLGGAHTISKLITEKLS---KLNGSEE	113	LKEKIEAAKKCSDDFTKKLQSSHAELGVAGGATTDENAKKAILKSNADKTKGADELGKLF	173	ESVESLAKAAKEMLANSVKELTSPVVAETPKX	205
SI-10	SAILMTLFLFISCNNSGKDGNSAST-NPVDESVKGPNLIEISKKITDSNAVVLAVQEVET	59	LLASIDEIAKKAIGQRIAPGNTLTADA-SRNGSLIVGVYTISTLITGKLN---GLKDLED	115	LKEKIEKAKKCSTEFTKKLLDSHAELGQADGAVTDDNAKRAILKTHNNTDKGAKELKDLS	175	ESVESLAKAAKEMLNNAVKELTKPVVAEAPKX	207
MOS-1b	SAILMTLFLFISCNNSGKDGNSAST-NPVDESVKGPNLIEISKKITDSNAVVLAVQEVET	59	LLASIDEIAKKAIGQRIAPGNTLTADA-SRNGSLIVGVYTISTLITGKLN---GLKDLED	115	LKEKIEKAKKCSTEFTKKLLDSHAELGQADGAVTDDNAKRAILKTHNNTDKGAKELKDLS	175	ESVESLAKAAKEMLNNAVKELTKPVVTEAPKX	207
21123	SAILMTLFLFISCNNSGKDGNSASN-NSADESVKGPNLVEISKKITDSNAVVLAVQEVET	59	LLASIDELAKKAIGQRIDQGNKLAVDA-DHNGSLLAGVYTISTLITEKLN---SLKISEE	115	LKEKIEKAKKYSTEFTKKLSDSHGVLGQVGGNATDVNAKAAILKTNQTTDKGAKELKELS	175	ESVESLAKAAKEMLNDSIKELTKPVVTEAPKX	207
BC-1	SAILMTLFLFISCNNSGKDGNSASN-NSADESVKGPNLIEISKKITDSNVVVLAVKEIET	59	LLSSIDELGNKAIGKLIS-ANGLNVQA-GQNGSLLAGAHAISSLITQKLS---ALN-SEE	113	LKEKIKDAKDCSEKFTKKLSESHADLGQN---ATDDNAKRAILKTHTDTDKGAKELKELS	170	ASVESLAKAAKELLNNAVKELTKPIVAESPKX	202
MI-6	SAILMTLFLFISCNNSGKDGNSAST-NPVDESVKGPNLTEISKKITDSNAVVLAVKEVET	59	LLASIDQLATKAIGKKIDQNNALGTQA-GQNGSLLAGAYTISTLITEKLSGLNALKNAEE	118	LKEKIKDAKKFSEDFTKKLSDNHADLSPA--AATDDNAKAAILKTNNTKDKGAKELKELS	176	ESVEALSKAAQAMLTNSVKELTSPVVAESPKX	208
MOD-1	SAILMTLFLFISCNNSGKDGNSAST-NPVDESVKGPNLTEISKKITDSNAVVLAVKEVET	59	LLASIDQLATKAIGKKIDQNNALGTQA-GQNGSLLAGAYTISTLITEKLSGLNALKNAEE	118	LKEKIKDAKKFSEDFTKKLSDSHADLSPA--AATDDNAKAAILKTNNTKDKGAKELKELS	176	ESVEALSKAAQAMLTNSVKELTSPVVAETPKX	208
831	SAILMTLFLFISCNNSGKDGN-TSA-NSADESVKGPNLTEISKKITDSNAVLLAVKEVEA	58	LLSSIDEIAAKAIGKKIHQNNGLDTEN-NHNGSLLAGAYAISTLIKQKLD---GLK-NEG	113	LKEKIDAAKKCSETFTNKLKEKHTDLGKE--GVTDADAKEAILKTNGTKTKGAEELGKLF	171	ESVEVLSKAAKEMLANSVKELTSPVVAESPKX	203
MI-2	SAILMTLFLFISCNNSGKDGN-TSA-NSADESVKGPNLTEISKKITDSNAVLLAVKEVEA	58	LLSSIDEIAAKAIGKKIHQNNGLDTEN-NHNGSLLAGAYAISTLIKQKLD---GLK-NEG	113	LKEKIDAAKKCSETFTNKLKEKHTDLGKE--GVTDADAKEAILKTNGTKTKGAEELGKLF	171	ESVEVLSKAAKEMLANSVKELTNPVVAESPKX	203
25015	SAILMTLFLFISCNNSGKDGNAAST-NPADESVKGPNLTEISKKITDSNAVVLAVKEVGA	59	LLTSIDELATKAIGKKIHQNNGLDTEN-NHNGSLLAGAYAISTLITQKLG---GLK-NEE	114	LKEKIAAVKKCSEEFTNKLKSSHTELGKQ--DAQDDDAKKAILRTHNTKDKGAEELDKLF	172	KAVENLSKAAKEMLSNSVKELTSPVVAESPKX	204
MI-5	SAILMTLFLFISCNNSGKDGNSAST-NPADESTKGPNLTEISKKITDSNAVVLAVKEVET	59	LIASIDELADKAIGKKIQQNNGLGTEN-NHNGSLLAGVYAISTLITQKLS---SLN-SEE	114	LKEKIKEAKDCSEKFTKKLETGHAELGKE--DATDDNAKKAVLKTNADKSKGAEELEKLF	172	KSVESLAKAAKESLNKSVKELTSPVVAESPKX	204
MI-9	SAILMTLFLFISCNNSGKDGNSAST-NPADESTKGPNLTEISKKITDSNAVVLAVKEVET	59	LIASIDELADKAIGKKIQQNNGLGTEN-NHNGSLLAGVYAISTLITQKLS---SLN-SEE	114	LKEKIKEAKDCSEKFTKKLETGHAELGKE--DATDDNAKKAVLKTNADKSKGAEELEKLF	172	KSVESLAKAAKESLNKSVKELTSPVVAKSPKX	204
FD-1	SAILMTLFLFISCNNSGKVGNSAST-NPADESTKGPNLTEISKKITDSNAVVLAVKEVET	59	LIASIDELADKAIGKKIQQNNGLGTEN-NHNGSLLAGAYAISTLITQKLS---KLN-SEE	114	LKEKIKEAKKCSEKFTKKLETGHAELGKE--DATDDNAKKAILKTNADKSKGAEELEKLF	172	KSVESLAKAAKEMLTNSVKELTSPVVAKNPKX	204
MOD-5	SAILMTLFLFISCNNSGKDGNSASN-NSADESAKGPNLIEISKKITDSNAVVLAVKEVET	59	LIASIDELA-KAIGKKIQQNNGLGTEN-NQNGSLLAGVHAISTLITEKLN---ALN-SET	113	LKEKIKEVKKCSENFTNTLKQNHTELGKK--DANDDDAKKAILRTNGDKTKGAKELEDLF	171	KAVESLAKAAKEILNNSVKELTSPVVGKNPKX	203
MOK-3a	SAILMTLFLFISCNNSGKDGNSAST-NPADESVKGPNLTEISKKITDSNAVVLAVKEVET	59	LIASIDELA-KAIGKKIEPTGGLGTDN-NHNGSLLSGAHAISTLITQKLS---ALN-SEE	113	LKGKIEDVKKCSQEFTNQLKNSHTELGKQ--DATDENAKKAILKTNAAKDKGAEELGELF	171	ESVESLSKAAKEMLANAVKELTSPVVAETPKX	203
SI-1	SAILMTLFLFISCNNSGKDGNASV--NSADESVKGPNLVEISKKITDSNAVVIAVKEVET	58	LLVSIDELA-KAIGKKIEAGGTLGSDG-AHNGSLLAGAYKIATEITANLS---KLKASED	113	LKEKITKAKECSEKFTDKLKSENAALGKQ--DASDDDAKKAILKTHNDITKGAKELKELS	171	ESVETLLKAAKEMLANSVKELTSPVVAKNPKX	203
SM-1	SAILMTLFLLISCNNSGKDGNASV--NSADESVKGPNLVEISKKITDSNAVVIAVKEVET	58	LLVSIDELA-KAIGKKIEAGGTLGSDG-AHNGSLLAGAYKIATEITANLS---KLKASED	113	LKEKITKAKECSEKFTDKLKSENAALGKQ--DASDDDAKKAILKTHNDITKGAKELKELS	171	ESVETLLKAAKEMLANSVKELTSPVVAKNPKX	203
SCI-2	SAILMTLFLFISCNNSGKDGNASV--NSADESVKGPNLVEISKKITDSNAVVIAVKEVET	58	LLVSIDELA-KAIGKKIEAGGTLGSDG-AHNGSLLAGAYKIATEITANLS---KLKASED	113	LKEKITKAKECSEKFTDLKSENAALGKQ--DASDDDAKKAILKTHNDITKGAKELKELS	171	ESVETLLKAAKEMLANSVKELTSPVVAKNPKX	203
SCW-30h	SAILMTLFLFISCNNSGKDGNSAS--NTADESVKGPNLTEISKKIADSNAVVLAVKEVEA	58	LLASIDELA-KAIGKKIKNDGSLEAEA-NRNESLVAGAYTISALITQKLG---KLKNSEE	113	LKEKISNAKDCAEAFTKKLKDNQAELGVQ--GVTDENSKKAILKTHGDKTKGAEELGKLF	171	ESVGNLSKAAKELLDNSVKELTSPVVAENPKX	203
AI-1	SAILMTLFLFISCNNSGKDGNSASTNNPADESTKGPNLTEISKKITDSNAVVLAVKEVVT	60	LIASIDELS-KAIGKKFNQNGGLDAEA-DHNGSLLSGAYTISTLITQKLG---KLKNSEE	115	LKEKIENAKKCSEEFTKKLKDNQADLGKK--DATDENAKKAILKTHGTTDKGAKELKELS	173	ESVEILIKAAKDMLTNSVKALTSPVVAENPKX	205
MI-8	SAILMTLFLFISCNNSGKDGNSAST-NPADESAKGPNLTEISKKITDSNTVVLAVKEVET	59	LLASIDELATKAIGKKIQQNGGLAAEA-GHNGTLLAGAYTISKLITQKLD---GLKNTEK	115	LKEIENAKKCSEDFTKKLEGEHVVLGIE--NVTDENAKKAILITDAAKDKGAAELEKLF	173	KSVESLAKAAQESLANSVKELTSPVVAENPKX	205
VS461	SAILMTLFLFISCNNSGKGGDIAST-NP-DESAKGPNLTEISKKITDSNAVVLAVKEVEA	58	LLSSIDELA-KTIGKKIEANG-LGNEA-DKNGSLLAGAYAISTLIKQKLD---GLKGLEG	112	LNKEIAEAKKCSEAFTKKLQDSNADLGKHN--ATDADSKEAILKTNGTKTKGAKELEELF	170	KSVESLSKAAKEALSNSVKELTSPVVAESPKX	202
20047	SAILMTLFLFISCNNS--GGDTAST-NP-DESVKGPNLTEISKKITDSNAFVLAVKEVEA	56	LISSIDELA-KAIGQRIQQNG-LVADA-GHNSALLAGAHEISILITQKLD---GLKGLEG	110	LKAEIAEAKKYSEAFTKKLKDNHAQLGIQNGASLDDEAKKAILKTNVDKTKGAEELEKLF	170	KSVESLSKAAQEALTNSVKELTNPVVAETPKX	202
VS116	SAILMTLFLFISCNNS--GGDTAST-NPVDESAKGPNLTEISKKITDSNAIVLAVKEVET	57	LLASINEIANKGIGKKINQNG-LDNLT-DHNGSLIAGAYVISTLITEKLN---NLKNSEG	112	LKEKIKKVKECSDKFTKKLTTSNGDLGKEN--VTDAHAQAAILKTNPTNDKGAKELGELF	170	ESVEILSKAAQEALTNSIAELTSPVVAENPKX	202
	*************** * * ******** ****** ******		* ** * ** * * * * * * * *		* * * ** * * ***** ** *		* * *** * **** ***

^aDashes indicate gaps. The conserved amino-terminal (C1), variable (V1 and V2), and semivariable (SV) domains and species-specific motif of the OspC proteins are indicated. Identical amino acid sequences (indicated by asterisks) of strains suggest a lateral genetic transfer of part of the ospC genes within and between genospecies.