TABLE 4.
Testing profiles of seven specimens found discordant when tested by two technologists in replicates of three in the LCx C. pneumoniae RUO assay
| Identification no. (dilution) | No. of positives/total no. of replicates tested by laboratory B in-house PCR | Assay valuea for the indicated replicate tested by:
|
|||||
|---|---|---|---|---|---|---|---|
| Technologist 1
|
Technologist 2
|
||||||
| 1 | 2 | 3 | 1 | 2 | 3 | ||
| T2219 (10−7) | 3/6 | 354.0 (+) | 55.3 (−) | 10.7 (−) | 9.4 (−) | 9.5 (−) | 9.6 (−) |
| VR-1310 (10−7) | 2/6 | 482.7 (+) | 782.8 (+) | 710.6 (+) | 9.5 (−) | 9.9 (−) | 9.8 (−) |
| A03 (10−7) | 2/6 | 9.7 (−) | 10.3 (−) | 10.0 (−) | 239.4 (+) | 9.6 (−) | 9.7 (−) |
| 379 CBD (10−6) | 2/6 | 540.7 (+) | 10.0 (−) | 9.7 (−) | 10.7 (−) | 10.3 (−) | 10.1 (−) |
| TNFαI 3I (10−5) | 1/6 | 11.8 (−) | 9.5 (−) | 11.9 (−) | 10.8 (−) | 10.6 (−) | 521.1 (+) |
| PS32 (10−7) | 0/6 | 10.0 (−) | 273.2 (+) | 10.4 (−) | 10.0 (−) | 9.8 (−) | 9.6 (−) |
| TNFαI 2I (10−5) | 0/6 | 366.9 (+) | 10.4 (−) | 11.3 (−) | 9.8 (−) | 9.6 (−) | 9.7 (−) |
a
The cutoff for positivity in the LCx RUO PCR is 100.0, and positive (+) and negative (−) results are indicated by symbols in parentheses.