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Predicted fragment sizes after digestion of the nox-specific PCR product with DpnII and BfmI for the five porcine Brachyspira spp.
| Species | Predicted restriction fragments (bp) obtained with:
|
|
|---|---|---|
| DpnII | BfmI | |
| B. hyodysenteriae | 684, 209, 24 | 741, 197 |
| B. pilosicoli | 896, 24 | 741, 197 |
| B. intermedia | 684, 209, 24 | 504, 238, 197 |
| B. innocens | 684, 209, 24 | 504, 210, 197, 25 |
| B. murdochii | 684, 157, 24 | 504, 210, 197, 25 |
Values in bold are fragments not visualized in ethidium bromide-stained gels.
