Fig. 3.

Effect of taurine on cysteine metabolism in liver. (A) Heat maps showing the differentially expressed genes (p < 0.05) which are related to cysteine metabolism in the liver between taurine-treated and control mice, as assessed by microarray previously performed. (B) CSAD and CTH were analyzed by Western blot and band intensity was calculated. The intensity was normalized by the GAPDH level. n = 4. ∗p < 0.05 (student's t-test). (C–F) Hepatic taurine, cystine, H₂S and sulfane sulfur were measured. Values were normalized by tissue weight (g). n = 8 (taurine, cysteine, H₂S), 4 (sulfane sulfur). ∗p < 0.05 (student's t-test). (G) Pathway of cysteine metabolism was shown. Red (increase) and blue (decrease) arrows indicate the effect.