TABLE 3.
Oligonucleotide primers and LightCycler hybridization probes used in the PCR assay
| Oligo- nucleotide | Sequencea | Target gene(s) | Nucleotide position | GenBank accession no. | Reference |
|---|---|---|---|---|---|
| STEC-1 | gA(Ag) C(Ag)A AAT AAT TTA TAT gTg | stx1 and stx2 | 280-300 | AB015056 | Present study |
| STEC-2 | TgA TgA Tg(Ag) CAA TTC AgT AT | stx1 and stx2 | 800-781 | AB015056 | 28 |
| STEC-I HP-1 | TTT ACg TTT TCg gCA AAT ACA gAg ggg AT-[FL] | stx1 | 570-598 | AB015056 | Present study |
| STEC-I HP-2 | [Red 640]-TCg TAC AAC ACT ggA TgA TCT CAg Tgg g-Ph | stx1 | 601-627 | AB015056 | Present study |
| STEC-II HP-1 | TCA ggC ACT gTC TgA AAC TgC TCC TgT gTA-[FL] | stx2 | 778-807 | Z37725 | Present study |
| STEC-II HP-2 | [Red 705]-ACC ATg ACg CCg ggA gAC gTg gAC CT-Ph | stx2 | 809-834 | Z37725 | Present study |
| eaeAF | gAC CCg gCA CAA gCA TAA gC | eae | 24875-24894 | AF022236 | 31 |
| eaeAR | CCA CCT gCA gCA ACA AgA gg | eae | 25258-25239 | AF022236 | 31 |
| eae-HP-1 | ACA gTT CTg AAA gCg AAA TgA TgA Agg C-[FL] | eae | 25132-25159 | AF022236 | Present study |
| eae-HP-2 | [Red 640]-CCT ggT CAg CAg ATC ATT TTg CCA CT-Ph | eae | 25164-25189 | AF022236 | Present study |
| hlyAF | gCA TCA TCA AgC gTA CgT TCC | E-hly | 70-90 | X94129 | 31 |
| hlyAR | AAT gAg CCA AgC Tgg TTA AgC T | E-hly | 602-581 | X94129 | 31 |
| hlyA HP-1 | gCA Tgg CTC TTg ATg AAT TgC TgA gA-[FL] | E-hly | 434-459 | X94129 | Present study |
| hlyA HP-2 | [Red 705]-CAA Cgg gAA ggA gAg gAT ATA AgT CAg-Ph | E-hly | 464-489 | X94129 | Present study |
a
[FL], fluorescein; [Red 640], LC Red 640-N-hydroxy-succinimide ester; [Red 705], LC Red 705-phosphoramidite; [Ph], 3′-phosphate. g = guanosine. A lowercase letter is used for this nucleotide to avoid confusion with C (cytosine) in the printed table.