TABLE 5.

Melting peak temperatures of the stx₂ LightCycler hybridization probes for 117 STEC strains whose stx₂ genes were previously characterized by block cycler PCR and PCR-RFLP

stx2 gene(s) present	No. of strains	Melting temp. (°C)	Serotype(s)a
stx2	27	71	O6:NM (1), O26:H11/NM (7), O103:H2 (1), O111:NM (3), O145:NM (1), O157:H7/NM (13), Ound:NM (1)
stx2c	18	71	O23:NM (1), O30:NM (1), O77:NM (1), O92:NM (1), O120:NM (1), O145:NM (2), O157:H7/NM (10), Orou:NM (1)
stx2 and stx2c	6	71	O157:H7 (6)
stx2d	39	55	O8:NM (1), O16:H32/H48 (2), O40:H6/NM (2), O62:NM (1), O70:NM (1), O75:H21 (1), O91:NM (1), O96:NM (1), O103:NM (1), O113:NM (2), O128:H2/NM (8), O140:NM (1), O146:NM (1), O162:NM (1), Orou:H19/NM (4), Ound:H8 (4), Ound:NM (7)
	1b	51	O22:H8 (1)
stx2e	15	66	O2:H5 (1), O8:Hund (1), O60:NM (2), O60:H2/NM (4), O101:H9 (1), Ound:NM (6)
	1c	63	Ound:NM (1)
stx2f	10	No melting	O15:NM (1), O26:NM (1), O66:NM (1), O83:H19 (1), O128:NM (3), O135:NM (1), Ound:NM (1), Ound:NM (1)
Total	117

^aOund and Hund, O and H antigen, respectively, not determined with available typing sera; Orou, O antigen not determined because of incomplete or rough lipopolysaccharide; NM, nonmotile. Numbers in parentheses indicate number of strains with that serotype.

^bNucleotide sequence variant of stx_2d.

^cNucleotide sequence variant of stx_2e.