TABLE 1.
Genome sequence details of B. cereus ciprofloxacin-exposed derivativesa
| Illumina data | ONT data | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Abx exp. no. | Modal MIC (ug/mL) |
Sample no. | SRA accession no. |
Total reads | SRA accession no. |
Total reads | ONT N50 | GenBank WGS accession no. | Assembly size (bp) | No. of contigs |
No. of CDSs | Depth of coverage (X) | Breadth of coverage |
| P1 | 0.125 | 1 | SRR31480868 | 1,702,794 | SRR31480826 | 147,552 | 7,306 | JBJJSR000000000 | 5,450,604 | 3 | 5,438 | 244 | 100 |
| C4 | 0.5 | 13 | SRR31480809-SRR31480814, SRR31480815-SRR31480822 | 937,901.5 ± 388,956.8 | SRR31480923-SRR31480924, SRR31480957-SRR31480963, SRR31480965-SRR31480968 | 87,786.0 ± 48,783.4 | 13,685.9 ± 6,084.6 | JBJJP[W-Z]000000000, JBJJQ[A-I]000000000 | 5,187,781.4 ± 48,861.0 | 4.1 ± 1.8 | 5,175.7 ± 45.2 | 217.7 ± 34.9 | 100.0 ± 0.0 |
| C6 | 2 | 3 | SRR31480806-SRR31480808 | 1,564,483.5 ± 739,312.0 | SRR31480919-SRR31480920, SRR31480922 | 109,370.5 ± 75,253.8 | 11,965.0 ± 7,861.6 | JBJJQ[J-L]000000000 | 5,1095,82.5 ± 74,682.5 | 7.0 ± 2.8 | 5,104.5 ± 74.2 | 228.5 ± 3.5 | 100.0 ± 0.0 |
| C7 | 2 | 5 | SRR31480800-SRR31480801, SRR31480803-SRR31480805 | 1,125,224.6 ± 332,287.8 | SRR31480914-SRR31480918 | 82,699.2 ± 53,570.3 | 10,787.6 ± 6,636.8 | JBJJQ[M-Q]000000000 | 5,059,125.4 ± 96,557.6 | 11.2 ± 7.8 | 5,067.6 ± 82.9 | 183.0 ± 79.4 | 100.0 ± 0.0 |
| C8 | 4 | 21 | SRR31480935-SRR31480937, SRR31480939-SRR31480948, SRR31480950-SRR31480956, SRR31480799 | 1,271,234.1 ± 424,687.7 | SRR31480861-SRR31480862, SRR31480893-SRR31480898, SRR31480900-SRR31480909, SRR31480911, SRR31480913 | 91,375.6 ± 59,276.7 | 12,533.0 ± 4,224.9 | JBJJQ[R-Z]000000000, JBJJR[A-L]000000000 | 5,061,076.9 ± 52,715.8 | 6.9 ± 2.9 | 5,062.6 ± 55.6 | 241.2 ± 50.5 | 100.0 ± 0.0 |
| C9 | 8 | 31 | SRR31480869-SRR31480872, SRR31480874-SRR31480883, SRR31480885-SRR31480892, SRR31480925-SRR31480926, SRR31480928-SRR31480934 | 1,064,004.1 ± 316,069.5 | SRR31480827-SRR31480835, SRR31480837-SRR31480846, SRR31480848-SRR31480857, SRR31480859-SRR31480860 | 99,457.8 ± 76,133.9 | 15,576.9 ± 8,038.4 | JBJJR[M-Z]000000000, JBJJS[A-Q]000000000 | 5,066,966.4 ± 68,586.6 | 8.4 ± 5.8 | 5,069.3 ± 71.8 | 241.4 ± 28.8 | 100.0 ± 0.0 |
| C13 | 16 | 7 | SRR31480938, SRR31480949, SRR31480802, SRR31480813, SRR31480824, SRR31480987-SRR31480988 | 1,217,210.4 ± 325,061.0 | SRR31480984-SRR31480985, SRR31480863-SRR31480867 | 117,176.0 ± 37,654.9 | 9,717.0 ± 3,846.2 | JBJJP[B-H]000000000 | 5,048,248.4 ± 13,234.9 | 5.4 ± 2.4 | 5,035.3 ± 4.3 | 268.3 ± 28.7 | 100.0 ± 0.0 |
| C15 | 16 | 2 | SRR31480927, SRR31480884 | 1,226,321.0 ± 101,181.3 | SRR31480982-SRR31480983 | 142,016.0 ± 15,289.1 | 6,991.0 ± 554.4 | JBJJP[I-J]000000000 | 5,017,976.0 ± 30,578.1 | 7.5 ± 2.1 | 4,979.0 ± 32.5 | 296.0 ± 2.8 | 100.0 ± 0.0 |
| C16 | 32 | 5 | SRR31480921, SRR31480964, SRR31480975, SRR31480986, SRR31480873 | 879,075.8 ± 107,032.1 | SRR31480977-SRR31480981 | 106,502.2 ± 31,171.0 | 10,664.0 ± 3,701.3 | JBJJP[K-O]000000000 | 5,075,772.4 ± 60,766.6 | 10.0 ± 3.2 | 5,069.4 ± 65.5 | 243.0 ± 52.2 | 100.0 ± 0.0 |
| C17 | 32 | 4 | SRR31480847, SRR31480858, SRR31480899, SRR31480910 | 917,156.5 ± 399,580.1 | SRR31480972-SRR31480976 | 160,114.2 ± 29,960.6 | 6,057.8 ± 1,697.5 | JBJJP[P-S]000000000 | 5,035,608.8 ± 47,085.5 | 11.2 ± 7.9 | 5,019.8 ± 59.8 | 229.0 ± 56.2 | 100.0 ± 0.0 |
| C20 | 32 | 3 | SRR31480823, SRR31480825, SRR31480836 | 1,083,012.0 ± 250,219.4 | SRR31480969-SRR31480971 | 228,769.3 ± 65,182.2 | 4,034.0 ± 1,162.9 | JBJJP[T-V]000000000 | 4,858,822.0 ± 156,534.1 | 20.7 ± 17.2 | 4,846.3 ± 158.2 | 182.7 ± 50.5 | 100.0 ± 0.0 |
a
Antibiotic exposure number (P1 = parental strain, C# = exposure passage number), modal MIC for all colonies sequenced, the number of colonies from each exposure (sample no), and sequencing statistics are provided.