FIG. 1.

(A) Alignment of the 3′ end of the 16S rRNA sequence of ITM 00-1026 with selected closely related mycobacterial 16S rRNA. The numbering of the region in the 16S rRNA corresponds to that of the M. ulcerans sequence (base pairs 1110 to 1522) (GenBank reference sequence). Only nucleotides that differ from the reference sequence are shown. Dashes indicate deletions or absent nucleotides. (B) Phylogenetic tree based on the alignments of the partial 16S rRNA gene sequences illustrating the position of isolate ITM 00-1026 in relation to those of other closely related mycobacteria. The tree was constructed by using the neighbor-joining method. Bootstrapping (1,000 replicates) was used to assess support for particular nodes in the tree, and the values are shown above the nodes.