TABLE 5.

Co-culture conditions of an 3D immune cell-containing tissue model.

	Murkar et al. (2024)
Cells and culture medium	Fibroblasts from fascia biopsies cultivated in DMEM suppl. with FBS. THP-1 cells cultivated in THP-1 medium (RPMI-1640, L-glutamine, FBS, penicillin/streptomycin) and PMA. Differentiation into M0, M1 and M2 macrophages using specifically supplemented THP-1 medium
3D cell culture technique	Collagen based scaffold derived from porcine intestine (SIS-muc) seeded with fibroblasts on the apical side. After 24 h, scaffolds were placed within cell crown inserts into medium (50:50 THP-1 medium and fibroblast medium) with differentiated macrophages (either M0, M1 or M2) and cultured for 11 days
Bacteria species	S. simulans and P. stutzeri mixed and cultivated for 48 h on polypropylene mesh (implant material) for biofilm formation
Bacterial challenge	Bacteria loaded polypropylene meshes placed on top of the fibroblasts Co-culture time 3 days
Sketch of the model	from Murkar et al. (2024)