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. 2002 Aug;40(8):2813–2822. doi: 10.1128/JCM.40.8.2813-2822.2002

TABLE 1.

PCR primers used in identification of resistance genes

Gene Oligonucleotide sequencesa Product size (bp) Reference or source
blaPSE1 F, TTT GGT TCC GCG CTA TCT G; R, TAC TCC GAG CAC CAA ATC CG 150 9
blaTEM F, GCA CGA GTG GGT TAC ATC GA; R, GGT CCT CCG ATC GTT GTC AG 310 9
aphA1-lab F, AAA CGT CTT GCT CGA GGC; R, CAA ACC GTT ATT CAT TCG TGA 500 14
aadA F, GTG GAT GGC GGC CTG AAG CC; R, AAT GCC CAG TCG GCA GCG 528 24
strA F, CTT GGT GAT AAC GGC AAT TC; R, CCA ATC GCA GAT AGA AGG C 548 AF273682
strB F, ATC GTC AAG GGA TTG AAA CC; R, GGA TCG TAG AAC ATA TTG GC 509 AF024602
sulA F, CAC TGC CAC AAG CCG TAA; R, GTC CGC CTC AGC AAT ATC 360 AF071555
tetA F, GCT ACA TCC TGC TTG CCT TC; R, CAT AGA TCG CCG TGA AGA GG 210 X61367
tetB F, TTG GTT AGG GGC AAG TTT TG; R, GTA ATG GGC CAA TAA CAC CG 659 19
tetG F, CAG CTT TCG GAT TCT TAC GG; R, GAT TGG TGA GGC TCG TTA GC 844 19
grm F, AAG CGC ACG AAG CGC GGG CTG; R, AAG GCG GGC CTC AAG GAG GTC 414 14
aadB F, GAG CGA AAT CTG CCG CTC TTG; R, CTG TTA CAA CGG ACT GGC CGC 310 14
aac(6)-I F, TGA GCA TGA CCT TGC GAT; R, GAA CAG CAA CTC AAC CAG 337 AF282595
int F, GGC ATC CAA GCA GCA AG; R, AAG CAG ACT TGA CCT GA Variable 19
a

F, forward; R, reverse.