Fig. 6.

Three-dimensional organization and PIA distribution of the vegetation formed by strains SA113, Newman HOM, and Newman D2C HOM in PS-based microplates. Exponential growth phase cells of S. aureus strains were inoculated into TSB supplemented with 0.5% glucose and cultured in tissue culture-treated, PS-based 6-well microplates for 18 h at 37 °C under static conditions. The vegetation formed were stained with XFD488-WGA and Nile Red (NR), and fluorescence was monitored with CLSM. (a,b) Three-dimensional image reconstructions of z series were recorded at 525 nm (XFD488) and 595 nm (NR), respectively. Reconstructed top view (a) and side view (b) images of the vegetation formed by the test strains. CLSM reconstructions are representative of three separate experiments. Each side of a grid square in the image reconstructions represents 100 μm. Scale bar, 200 μm (scale bar applies to all images in the respective panel).