Table 2.
Spearman correlations of the Microbiome and parameter changes between timepoints 6 h and 24 h after Bruce intervention. Only correlations with significant p-values (< 0.05) are included.
| Group | Parameter | Microbiome feature | Spearman correlation | Adjusted p value |
|---|---|---|---|---|
| Control | Leptin [pg/ml] | PWY0-1241: ADP-l-glycero-β-d-manno-heptose biosynthesis | 0.95 | < 0.001 |
| LIF [pg/ml] | RIBOSYN2-PWY: flavin biosynthesis I (bacteria and plants) | 0.90 | 0.02 | |
| IL-6 [pg/ml] | Bacteroides cellulosilyticus | − 0.88 | 0.03 | |
| LIF [pg/ml] | PWY-5971: palmitate biosynthesis (type II fatty acid synthase) | − 0.87 | 0.06 | |
| Il-10 [pg/ml] | PWY0-1241: ADP-l-glycero-β-d-manno-heptose biosynthesis | − 0.83 | 0.10 | |
| LIF [pg/ml] | Bacteroides caccae | − 0.84 | 0.11 | |
| LIF [pg/ml] | Clostridiaceae bacterium | 0.80 | 0.12 | |
| LIF [pg/ml] | BIOTIN-BIOSYNTHESIS-PWY: biotin biosynthesis I | − 0.80 | 0.12 | |
| LIF [pg/ml] | FASYN-ELONG-PWY: fatty acid elongation—saturated | − 0.79 | 0.12 | |
| LIF [pg/ml] | PWY-7664: oleate biosynthesis IV (anaerobic) | − 0.79 | 0.12 | |
| LIF [pg/ml] | PWY-6609: adenine and adenosine salvage III | 0.83 | 0.12 | |
| LIF [pg/ml] | PWY-6519: 8-amino-7-oxononanoate biosynthesis I | − 0.79 | 0.12 | |
| LIF [pg/ml] | PWY0-862: (5Z)-dodecenoate biosynthesis I | − 0.79 | 0.12 | |
| LIF [pg/ml] | PWY-6282: palmitoleate biosynthesis I (from (5Z)-dodec-5-enoate) | − 0.79 | 0.12 | |
| Leptin [pg/ml] | Roseburia faecis | − 0.82 | 0.15 | |
| LIF [pg/ml] | PWY-6572: chondroitin sulfate degradation I (bacterial) | − 0.78 | 0.15 | |
| FSTL1 [pg/ml] | PWY0-1241: ADP-l-glycero-β-d-manno-heptose biosynthesis | − 0.80 | 0.16 | |
| Il-10 [pg/ml] | PWY-6700: queuosine biosynthesis I (de novo) | 0.80 | 0.17 | |
| LIF [pg/ml] | PWY-8187: L-arginine degradation XIII (reductive Stickland reaction) | − 0.76 | 0.18 | |
| SPARC [pg/ml] | PWY0-1241: ADP-l-glycero-β-d-manno-heptose biosynthesis | − 0.80 | 0.18 | |
| Strength | IL-15 [pg/ml] | PWY0-1298: superpathway of pyrimidine deoxyribonucleosides degradation | − 0.95 | < 0.02 |
| IL-10 [pg/ml] | Clostridium sp AF27 2AA | 0.92 | 0.10 | |
| IL-6 [pg/ml] | Gemmiger formicilis | 0.92 | 0.11 | |
| Endurance | IL-15 [pg/ml] | Eubacterium rectale | − 0.95 | < 0.001 |
| TfR [pg/ml] | Blautia wexlerae | − 0.93 | < 0.01 | |
| TIMP1 [pg/ml] | Bacteroides thetaiotaomicron | 0.92 | < 0.01 | |
| TIMP1 [pg/ml] | PWY-5188: tetrapyrrole biosynthesis I (from glutamate) | − 0.92 | < 0.01 | |
| Adiponectin [pg/ml] | PWY3O-4107: NAD salvage pathway V (PNC V cycle) | − 0.92 | < 0.01 | |
| Adiponectin [pg/ml] | PWY-4041: γ-glutamyl cycle | − 0.90 | < 0.01 | |
| TIMP1 [pg/ml] | Blautia wexlerae | − 0.90 | < 0.01 | |
| Adiponectin [pg/ml] | COLANSYN-PWY: colanic acid building blocks biosynthesis | − 0.89 | < 0.01 | |
| TIMP1 [pg/ml] | GLYCOGENSYNTH-PWY: glycogen biosynthesis I (from ADP-D-Glucose) | − 0.88 | < 0.01 | |
| TIMP1 [pg/ml] | PWY-5030: l-histidine degradation III | 0.88 | < 0.01 | |
| TIMP1 [pg/ml] | HISDEG-PWY: l-histidine degradation I | 0.88 | < 0.01 | |
| TIMP1 [pg/ml] | Odoribacter splanchnicus | − 0.88 | < 0.01 | |
| TIMP1 [pg/ml] | Bacteroides uniformis | 0.84 | < 0.01 | |
| TIMP1 [pg/ml] | Dorea longicatena | 0.84 | < 0.01 | |
| TIMP1 [pg/ml] | Lacrimispora celerecrescens | − 0.84 | < 0.01 | |
| TIMP1 [pg/ml] | OANTIGEN-PWY: O-antigen building blocks biosynthesis (E. coli) | − 0.84 | < 0.01 | |
| TIMP1 [pg/ml] | DAPLYSINESYN-PWY: l-lysine biosynthesis I | − 0.83 | < 0.01 | |
| TIMP1 [pg/ml] | CITRULBIO-PWY: L-citrulline biosynthesis | 0.83 | < 0.01 | |
| TfR [pg/ml] | KETOGLUCONMET-PWY: ketogluconate metabolism | − 0.82 | 0.12 | |
| TfR [pg/ml] | PWY-5188: tetrapyrrole biosynthesis I (from glutamate) | − 0.88 | 0.12 | |
| TIMP1 [pg/ml] | PWY-7199: pyrimidine deoxyribonucleosides salvage | 0.78 | 0.16 | |
| TIMP1 [pg/ml] | NONOXIPENT-PWY: pentose phosphate pathway (nonoxidative branch) I | − 0.78 | 0.16 | |
| TIMP1 [pg/ml] | Eubacterium rectale | − 0.78 | 0.16 | |
| TIMP1 [pg/ml] | HSERMETANA-PWY: l-methionine biosynthesis III | − 0.78 | 0.16 | |
| TIMP1 [pg/ml] | P441-PWY: superpathway of N-acetylneuraminate degradation | − 0.84 | 0.17 | |
| TIMP1 [pg/ml] | ARGSYNBSUB-PWY: l-arginine biosynthesis II (acetyl cycle) | − 0.77 | 0.17 | |
| TIMP1 [pg/ml] | METH-ACETATE-PWY: methanogenesis from acetate | − 0.77 | 0.17 | |
| TIMP1 [pg/ml] | ARGSYN-PWY: l-arginine biosynthesis I (via l-ornithine) | − 0.77 | 0.17 | |
| TfR [pg/ml] | PWY-5030: l-histidine degradation III | 0.84 | 0.18 | |
| TfR [pg/ml] | Eubacterium rectale | − 0.84 | 0.18 | |
| TIMP1 [pg/ml] | PWY-7237: myo-, chiro- and scyllo-inositol degradation | − 0.76 | 0.19 | |
| TIMP1 [pg/ml] | P41-PWY: pyruvate fermentation to acetate and (S)-lactate I | − 0.76 | 0.19 | |
| TIMP1 [pg/ml] | PWY-6823: molybdopterin biosynthesis | − 0.76 | 0.19 |
TIMP1 (tissue inhibitor of metalloproteinases), IL-5 (interleukin 5), IL-6 (interleukin 6), IL-10 (interleukin 10), IL-15 (interleukin15), LIF (leukemia inhibitory factor), SPARC (secreted protein acidic and cysteine rich), TfR (transferrin receptor). Significant correlations (p-value < 0.05) were observed across all groups. In the control group, ADP-L-glycero-β-D-manno-heptose biosynthesis was positively correlated with leptin, while Bacteroides cellulosilyticus showed negative correlations with IL-6. The strength group revealed strong associations betweenof pyrimidine deoxyribonucleosides degradation and IL-15. In the endurance group, notable correlations included Eubacterium rectale with IL-15, and Blautia wexlerae with TfR, as well as various pathways involved in NAD (Nicotinamide Adenine Dinucleotide) salvage and histidine degradation. These findings highlight the dynamic microbiome-blood marker interactions induced by the Bruce test across different exercise modalities.
Significant values are in bold.