TABLE 1.
PCR, culture, and DFA assay results
| Test | No. of specimens with resulta
|
No. of adequate specimens with resultb
|
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Indeterminate | Positive | Negative | Missing | Total | Indeterminate | Positive | Negative | Missing | Total | |
| Culture | 0 | 10 | 215 | 0 | 225 | 0 | 9 | 170 | 0 | 179 |
| DFA | 0 | 4 | 182 | 39 | 186 | 0 | 4 | 150 | 25 | 154 |
| Agarose gel | ||||||||||
| Pertussis PCR | 0 | 10 | 215 | 0 | 225 | 0 | 10 | 169 | 0 | 179 |
| Globin PCR | 0 | 178 | 47 | 0 | 225 | 0 | 178 | 1c | 0 | 179 |
| Dot blot | ||||||||||
| Short exposure | 0 | 29 | 196 | 0 | 225 | 0 | 29 | 150 | 0 | 179 |
| Long exposure | 10 | 29 | 186 | 0 | 225 | 10 | 29 | 140 | 0 | 179 |
| Combined result | 1 | 30 | 194 | 0 | 225 | 1 | 30 | 148 | 0 | 179 |
a
Results are for all specimens.
b
Globin or pertussis amplicons were detected with multiplexed primers or with a globin primer pair alone.
c
This specimen was negative for pertussis and globin PCR amplicons by gel electrophoresis but positive for pertussis by dot blot hybridization.