TABLE 2.
Multiplex PCR identification of 286 positive blood cultures by comparison of organism PCR with direct PCR testing from clinical specimens
| Organism type | No. of strains | Expected PCR resultsa (16S/mecA/nuc) | Direct PCR results, 16S/mecA/nuc (no. of isolates) |
|---|---|---|---|
| CoNS,b methicillin resistant | 107 | +/+/− | +/+/− (100); −/+/− (7) |
| CoNS, methicillin susceptible | 73 | +/−/− | +/−/− (71); −/−/− (2) |
| MSSA | 36 | +/−/+ | +/−/+ (34); −/−/+ (2) |
| MRSA | 17 | +/+/+ | +/+/+ (15); −/+/+ (2) |
| Micrococcus spp. | 3 | +/−/− | +/−/− (3) |
| Escherichia coli | 12 | +/−/− | +/−/− (10); −/−/− (2) |
| Enterobacter cloacae | 3 | +/−/− | +/−/− |
| Serratia marcescens | 4 | +/−/− | +/−/− |
| Pseudomonas aeruginosa | 3 | +/−/− | +/−/− |
| Haemophilus influenzae | 2 | +/−/− | +/−/− |
| Neisseria meningitidis | 2 | +/−/− | +/−/− |
| Bacteroides fragilis | 1 | +/−/− | +/−/− |
| Streptococcus pyogenes | 2 | +/−/− | +/−/− |
| Streptococcus agalactiae | 5 | +/−/− | +/−/− |
| Enterococcus faecalis | 10 | +/−/− | +/−/− |
| Enterococcus faecium | 5 | +/−/− | +/−/− |
| Propionibacterium acnes | 1 | +/−/− | +/−/− |
a
+, presence of PCR product; −, absence of PCR product.
b
CoNS, coagulase negative staphylococci.