TABLE 3.

Discrepant results of direct PCR testing for 15 positive blood cultures compared with organism PCR^a

Organism type (no. of isolates)	Organism PCR (16S/mecA/nuc)	Direct PCR
		Neat (16S/mecA/nuc)	1:10 diluted template (16S/mecA/nuc)
MRSA (2)	+/+/+	−/+/+	+/+/+
MSSA (2)	+/−/+	−/−/+	+/−/+
MRCoNS (7)	+/+/−	−/+/−	+/+/−
MSCoNS (2)	+/−/−	−/−/−	+/−/−
E. coli (2)	+/−/−	−/−/−	+/−/−

^a+, presence of PCR product; −, absence of PCR product. MRCoNS and MSCoNS, methicillin-resistant and susceptible, coagulase-negative staphylococci, respectively.