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QTD PCR applied to cloned template: correlation between measured and calculated concentrations of P. carinii DHFR gene copies using serial dilutions of plasmid DNA. Values represent the mean (+1.96 standard deviations [SD]) calculated concentrations (⧫) of standards (5 to 500,000 copies/tube) assayed by QTD PCR (r = 0.99; n = 94).
