TABLE 1.
Percentages of isolates determined to be fluconazole susceptible, susceptible-dose dependent, and resistant among the 793 Candida spp. tested as determined by the NCCLS microdilution broth method and six commercial systemsa
| Method and species (no. of isolates) | % of isolates
|
||
|---|---|---|---|
| Susceptible | Susceptible-dose dependent | Resistant | |
| Microdilution broth | |||
| All isolates | 83.61 | 8.07 | 8.32 |
| C. albicans (478) | 90.1 | 3.2 | 6.7 |
| Non-C. albicans (315) | 74.0 | 15.7 | 10.3 |
| Sensititre YeastOne | |||
| All isolates | 81.69 | 12.94 | 5.46 |
| C. albicans (478) | 93.1 | 3.6 | 3.4 |
| Non-C. albicans (315) | 64.1 | 27.2 | 8.7 |
| Etest | |||
| All isolates | 89.39 | 3.91 | 6.69 |
| C. albicans (478) | 93.1 | 1.0 | 5.9 |
| Non-C. albicans (315) | 83.8 | 8.3 | 8.0 |
| Disk diffusion | |||
| All isolates | 82.35 | 10.34 | 7.31 |
| C. albicans (478) | 96.7 | 2.1 | 1.3 |
| Non-C. albicans (315) | 60.6 | 22.9 | 16.5 |
| Fungitest | |||
| All isolates | 86.29 | 9.01 | 4.70 |
| C. albicans (478) | 92.6 | 5.3 | 2.1 |
| Non-C. albicans (315) | 76.6 | 14.7 | 8.7 |
| Candifast | |||
| All isolates | 14.54 | 17.07 | 68.39 |
| C. albicans (478) | 6.1 | 17.4 | 76.5 |
| Non-C. albicans (315) | 27.4 | 16.6 | 56.1 |
| Integral System Yeasts | |||
| All isolates | 35.69 | 57.76 | 6.56 |
| C. albicans (478) | 40.6 | 58.2 | 1.3 |
| Non-C. albicans (315) | 28.3 | 57.1 | 14.6 |
Results of the reference method, Etest, Sensititre YeastOne, and disk diffusion were classified as susceptible, susceptible-dose dependent, or resistant according to the MIC breakpoints recommended for fluconazole in the M27-A protocol (7). For the remaining systems tested, the isolates were classified as susceptible, intermediate, or resistant in accordance with the individual manufacturer's instructions. For the purpose of analysis, the isolates classified with the conceptually similar term intermediate by Fungitest, Candifast, and Integral System Yeasts were included in the susceptible-dose dependent category.