Table 2.

Description of types of mutation templates tested.

Mutation template type	Description	# of templates
Primer-SNP	Single Nucleotide Polymorphism (SNP) within one of the primer regions, no mutation on any other component	91
Primer-NSNP	Non-SNP mutations (i.e., deletion, multi-nucleotide substitution) within one of the primer region, no mutation on any other component	58
Probe-SNP	SNP within the probe region, no mutation on any other component	34
Probe-NSNP	Non-SNP mutations (i.e., deletion, multi-nucleotide substitution) within the probe region, no mutation on any other component	10
MC	Multicomponent Mutations on more than one PCR component	35