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PCR cycling conditions.
| Step | Temperature (°C) | Time | Cycles |
|---|---|---|---|
| Reverse transcription | 50 | 15 min | 1 |
| Denaturation | 95 | 2 min | 1 |
| Annealing/extension | 95 | 3/5* s | 45*/50 |
| 55 | 30 s |
*CDC N1 and N2 templates were amplified with slightly altered melting time and total cycle number. For the purpose of this study, we have analyzed all the qPCR results together.
