Table 5.

Scheme for making actionable calls of PCR results.

	50 Copies/reaction	500 Copies/reaction	5000 Copies/reaction	50,000 Copies/reaction	Template label
Mutated Template 1	Significantly changed	Not significantly changed	Not significantly changed	Not significantly changed	Not significantly changed
Mutated Template 2	Significantly changed	Significantly changed	Significantly changed	Not significantly changed	Significantly changed
Mutated Template 3	Significantly changed	Significantly changed	Not significantly changed	Not significantly changed	Significantly changed