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Number of significantly changed templates according to 4 different thresholds among 5 different mutation template type.
| Primer-SNP | Primer-non-SNP | Probe-SNP | Probe-non-SNP | MC | |
|---|---|---|---|---|---|
| No detection (ND) | 0/77 | 7/58 | 0/48 | 5/10 | 6/35 |
| ΔCt > 1 or ND | 41/77 | 50/58 | 18/48 | 9/10 | 30/35 |
| ΔCt > 3 or ND | 20/77 | 38/58 | 1/48 | 8/10 | 23/35 |
| ΔCt > 5 or ND | 17/77 | 28/58 | 0/48 | 5/10 | 18/35 |
