TABLE 3.
Agreement between the NCCLS method and the Sensititre or the Etest method for each of five Aspergillus species for amphotericin B and itraconazole after 24 and 48 h of incubation
| Drug | Incubation time (h) | Species | % Agreement for Etest method
|
P value for Etest method | % Agreement for Sensititre method
|
P value for Sensititre method | ||
|---|---|---|---|---|---|---|---|---|
| ± 1 log2 dilution | ± 2 log2 dilution | ± 1 log2 dilution | ± 2 log2 dilution | |||||
| Itraconazolea | 24 | A. fumigatus | 82.0 ± 18.5c | 93.3 ± 14.8 | 2.2 ± 8.1 | 12.0 ± 7.1 | <0.05 | |
| A. flavus | 43.9 ± 15.6 | 82.7 ± 14.8 | 16.7 ± 8.1 | 72.5 ± 7.7 | <0.01 | |||
| A. nidulans | 58.9 ± 22.3 | 92.9 ± 13.5 | 4.8 ± 8.9 | 48.2 ± 12.0 | <0.01 | |||
| A. terreus | 53.5 ± 8.7 | 83.0 ± 10.0 | 21.1 ± 15.0 | 50.0 ± 27.9 | <0.01 | |||
| A. ustus | 2.6 ± 4.9 | 3.8 ± 7.3 | <0.001 | NCe | NC | |||
| Overall | 45.7 ± 36.1d | 72.5 ± 38.9 | <0.05 | 9.7 ± 10.3 | 44.6 ± 29.1 | <0.01 | ||
| 48 | A. fumigatus | 53.4 ± 13.0 | 62.8 ± 25.5 | <0.01 | 63.3 ± 3.3 | 96.6 ± 6.7 | <0.01 | |
| A. flavus | 17.2 ± 14.4 | 51.5 ± 8.9 | <0.01 | 50.2 ± 10.0 | 90.4 ± 5.7 | <0.01 | ||
| A. nidulans | 33.2 ± 8.7 | 73.0 ± 19.1 | 34.9 ± 5.7 | 86.8 ± 3.4 | <0.01 | |||
| A. terreus | 45.0 ± 5.7 | 81.6 ± 16.2 | 66.7 ± 6.4 | 96.7 ± 6.5 | <0.05 | |||
| A. ustus | 9.0 ± 8.8 | 12.6 ± 9.1 | <0.01 | 23.9 ± 18.2 | 32.8 ± 16.9 | <0.05 | ||
| Overall | 30.0 ± 21.2 | 56.0 ± 30.9 | 47.6 ± 20.8 | 84.6 ± 24.1 | ||||
| Amphotericin Bb | 24 | A. fumigatus | 91.8 ± 3.4 | 97.8 ± 4.3 | 77.3 ± 7.9 | 93.9 ± 13.4 | <0.05 | |
| A. flavus | 16.6 ± 3.2 | 40.0 ± 5.7 | <0.01 | 93.9 ± 13.4 | 97.2 ± 5.4 | |||
| A. nidulans | 37.9 ± 20.7 | 78.3 ± 19.7 | 93.0 ± 15.7 | 100.0 ± 0.0 | ||||
| A. terreus | 88.9 ± 8.2 | 99.4 ± 2.2 | 97.0 ± 5.8 | 100.0 ± 0.0 | ||||
| A. ustus | 95.0 ± 16.7 | 99.4 ± 2.4 | NC | 0.0 ± 0.0 | ||||
| Overall | 69.7 ± 37.3 | 89.2 ± 22.2 | <0.05 | 91.4 ± 8.7 | 98.9 ± 2.9 | |||
| 48 | A. fumigatus | 33.3 ± 6.4 | 45.0 ± 9.7 | <0.01 | 100.0 ± 0.0 | 100.0 ± 0.0 | ||
| A. flavus | 2.2 ± 4.3 | 5.0 ± 0.0 | <0.01 | 96.7 ± 6.5 | 99.4 ± 2.2 | |||
| A. nidulans | 34.8 ± 10.0 | 40.0 ± 0.0 | <0.05 | 92.0 ± 14.6 | 100.0 ± 0.0 | |||
| A. terreus | 16.4 ± 6.8 | 26.4 ± 8.5 | <0.01 | 100.0 ± 0.0 | 100.0 ± 0.0 | |||
| A. ustus | 75.1 ± 5.7 | 97.8 ± 4.3 | 99.4 ± 2.2 | 99.4 ± 2.2 | ||||
| Overall | 29.3 ± 30.9 | 43.6 ± 41.5 | 98.8 ± 3.0 | 99.9 ± 0.3 | ||||
MIC-2 was used as the endpoint for the NCCLS method, and MIC-P was used as the endpoint for the Sensititre method.
MIC-0 was used as the endpoint for the NCCLS method, and MIC-B was used as the endpoint for the Sensititre method.
For each of the five Aspergillus species, the average percent agreement and the 95% confidence intervals among the three replicates were calculated after angular transformation.
For the overall result, the average percent agreement and the 95% confidence intervals among the five Aspergillus species were calculated after angular transformation.
NC, not calculated.