TABLE 4.
Overall agreement for all 25 Aspergillus strains between the different MIC endpoints of the NCCLS, Sensititre, and Etest methods
| Incubation time (h) | Druga | NCCLS endpointb | % Agreement with 1 twofold dilution (2 twofold dilutions)
|
|||
|---|---|---|---|---|---|---|
| Etest method | Sensititre method
|
|||||
| MIC-I | MIC-R | MIC-P | MIC-B | |||
| 24 | ICZ | MIC-0 | 46.7 (64.5)c | 3.3 (4.4) | 3.5 (7.7) | 7.1 (22.6) |
| MIC-1 | 49.5 (70.2) | 4.4 (7.5) | 6.0 (25.3) | 18.0 (61.0) | ||
| MIC-2 | 48.1 (70.2) | 7.5 (14.1) | 11.9 (46.2) | 39.1 (74.8) | ||
| MIC-3 | 43.2 (59.9) | 9.8 (35.7) | 34.3 (71.5) | 66.7 (84.3) | ||
| MIC-4 | 27.8 (47.4) | 38.0 (70.4) | 69.6 (85.9) | 73.9 (86.8) | ||
| AMB | MIC-0 | 64.2 (81.9) | 11.2 (62.0) | 60.8 (91.7) | 87.9 (96.9) | |
| 48 | ICZ | MIC-0 | 70.4 (83.1) | 23.6 (26.0) | 25.7 (41.3) | 29.1 (60.9) |
| MIC-1 | 42.9 (66.8) | 16.6 (27.8) | 27.4 (67.6) | 42.6 (82.3) | ||
| MIC-2 | 32.2 (55.7) | 21.4 (50.3) | 48.0 (80.1) | 63.5 (84.6) | ||
| MIC-3 | 21.5 (37.9) | 41.3 (72.2) | 68.2 (82.8) | 75.4 (82.4) | ||
| MIC-4 | 15.0 (21.5) | 66.9 (81.5) | 68.7 (80.8) | 62.2 (76.1) | ||
| AMB | MIC-0 | 32.6 (42.7) | 45.6 (90.3) | 89.6 (99.3) | 96.4 (99.6) | |
| 24 and 48d | ICZ | MIC-0 | 54.1 (60.0) | 17.6 (23.6) | 22.8 (55.1) | 31.7 (74.7) |
| MIC-1 | 51.4 (64.5) | 19.0 (46.9) | 44.2 (75.7) | 59.4 (81.2) | ||
| MIC-2 | 48.3 (68.8) | 32.5 (61.6) | 56.6 (79.5) | 66.6 (80.2) | ||
| MIC-3 | 46.9 (64.0) | 51.1 (72.6) | 66.2 (78.9) | 66.5 (76.8) | ||
| MIC-4 | 37.6 (54.6) | 65.8 (79.2) | 52.1 (73.4) | 41.0 (63.9) | ||
| AMB | MIC-0 | 54.4 (67.9) | 86.6 (98.4) | 92.2 (99.2) | 84.3 (99.3) | |
a
ICZ, itraconazole; AMB, amphotericin B.
b
See footnote a of Table 2 for the definitions of the endpoints.
c
The 95% confidence intervals of the average percent agreement among the three replicates (for each replicate, 100 comparisons [five strains × five species × four observers] were calculated after angular transformation and ranged from ±0.9 to ±11.9% (median, 5%).
d
For the Etest method, the values are for the Etest method at 24 h and the NCCLS method at 48 h; and for the sensititre method, the values are for the NCCLS method at 24 h and the sensititre method at 48 h.