Table 2.

Descriptive statistics of qRT-PCR and ddPCR for all assays with lower limits of quantification (LLOQ).

Data sets	Statistical indicators	NCFM		Lpc-37		Bl-04
		qRT-PCR [genomes/10 ng]	ddPCR [copies/μl]	qRT-PCR [genomes/10 ng]	ddPCR [copies/μl]	qRT-PCR [genomes/10 ng]	ddPCR [copies/μl]
LLOQ (Lower Limit of Quantification)		100 fg	10 fg	100 fg	1 fg	100 fg	10 fg
		46.2	0.07	27.8	0.2	47.5	0.1
All Data (n = 248)	Min	24.9	0.5	20.7	0.3	26.2	0.4
	Max	155335.9	1767.0	31026.4	2564.0	219137.8	122.6
	Mean	10407.5	107.0	1160.6	123.7	3836.4	13.0
	Std. Dev.	29203.9	301.6	2914.7	284.4	26515.4	20.1
	n	56	40	141	194	67	50
True Positives (n = 60)	Min	29.3	0.5	21.7	0.4	26.2	0.4
	Max	10063.5	45.7	11879.3	2050.0	4991.7	122.6
	Mean	1159.5	7.7	1172.9	181.1	680.2	13.9
	Std. Dev.	2134.9	11.7	1698.5	295.9	933.4	20.9
	n	24	19	56	57	49	45
True Negatives (n = 188)	Min	24.9	0.6	20.7	0.3	27.5	0.7
	Max	155335.9	1767.0	31026.4	2564.0	219137.8	17.0
	Mean	17343.5	196.8	1152.5	99.8	12428.2	5.5
	Std. Dev.	37105.9	395.1	3491.6	276.0	50136.4	6.2
	n	32	21	85	137	18	5

Assays were quantifiable above the LLOQ and below this, assigned a value of half the LLOQ.