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. 2025 May 7;12:891–908. doi: 10.2147/JHC.S516656

Figure 6.

Figure 6

CA9-mediated the oncogenic phenotypes of DDX11-AS1 in HCC cells was confirmed by rescue experiments. (A) Correlation analysis between the expression of DDX11-AS1 and CA9 in HCC based on TCGA data. The regulatory effect of DDX11-AS1 on CA9 was validated by qRT-PCR (B) and Western blotting (C). Overexpression of CA9 attenuates the suppressive effects of DDX11-AS1 knockdown on the capacities of cell proliferation (D), colony formation (E), migration (F), and invasion (G). siNC, negative control; siDDX11-AS1, DDX11-AS1 knockdown; OE-NC, empty overexpression vector; OE-CA9, CA9 overexpression. Scale bar = 100 μm. *p < 0.05, **p < 0.01.