Fig. 3.

Mapping of the major RNAs produced from the yrzI locus. (A) Northern blots showing expression of the yrzI transcript in WT, ∆rae1 and plasmid complemented strains grown in the absence (−) and presence (+) of IPTG. Blots were probed with oligonucleotides complementary to yrzI (CC1589; green dot), S1027 (CC1598; purple dot) and S1024 (CC1600; gray dot) as indicated. Blots were rehybridized with a probe complementary to 16S rRNA (oligo CC058) as a loading control. This experiment was repeated twice. (B) Structure of the yrzI operon and summary of transcripts identified in panel (A). ORFs are represented by gray arrows, the putative promoters by rightward‐pointing arrows and putative transcription terminators by the hairpin structures. The sizes of the ORFs are given in amino acids (aa) and the lengths of the intergenic regions in nucleotides (nts) are indicated. The position of the 3 probes used is indicated by colors bars: purple for S1027, green for yrzI and gray for S1024. Transcripts from this locus are shown as wavy lines and those sensitive to Rae1 are in red. The presence or absence of the different species in the Northern blots shown in panel A is represented by (+) or (−) signs, respectively.