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Sensitivity levels of the in-house PCR assay with detection of amplicons by using an ethidium bromide-stained polyacrylamide gel and DEIA. Lanes: L, 100-bp ladder; 1 through 7, respectively, 105 to 10−1 M. tuberculosis CFU per ml of saline; 8, negative control. +, DEIA positive; −, DEIA negative.
