TABLE 1.
Vaginal isolates derived from 23 women by the different genotypic approaches
| Subject | RAPD type | Species designation by:
|
||
|---|---|---|---|---|
| TTGEa | Multiplex PCRb | Sequencing | ||
| A | 15 | >1 bandc | L. crispatus | L. crispatus |
| B | 21 | L. jensenii | L. jensenii | NDd |
| 15 | >1 band | L. crispatus | L. crispatus | |
| C | 10 | L. crispatus | L. crispatus | ND |
| D | 4 | L. gasseri | L. gasseri | ND |
| E | 1 | L. gasseri | L. gasseri | ND |
| F | 3 | L. gasseri | L. gasseri | ND |
| Ge | 10 | >1 band | L. crispatus | L. crispatus |
| 12 | >1 band | L. crispatus | L. crispatus | |
| He | 9 | >1 band | L. crispatus | L. crispatus |
| 16 | >1 band | L. crispatus | L. crispatus | |
| I | 19 (L. iners) | L. iners | ND | ND |
| J | 6 (L. jensenii)f | L. jensenii | L. jensenii | ND |
| K | 17 | L. crispatus | L. crispatus | ND |
| L | 11 | L. gasseri | L. gasseri | ND |
| M | 5 | L. gasseri | L. gasseri | ND |
| 8 | L. crispatus | L. crispatus | ND | |
| N | 7 | >1 band | L. crispatus | L. crispatus |
| O | 19 (L. iners) | L. iners | ND | ND |
| P | 14 | L. gasseri | L. gasseri | ND |
| Q | 8 | >1 band | L. crispatus | L. crispatus |
| R | 6 (L. jensenii) | L. jensenii | L. jensenii | ND |
| S | 7 | >1 band | L. crispatus | L. crispatus |
| T | 19 (L. iners) | L. iners | ND | ND |
| U | 20 (L. iners) | L. iners | ND | ND |
| V | 10 | >1 band | L. crispatus | L. crispatus |
| 3 | L. jensenii | L. jensenii | ND | |
| W | 2 | L. gasseri | L. gasseri | ND |
a
See Fig. 1.
b
The isolates were grouped first by PCR-G and then identified to the species level by PCR II-1 and PCR II-2 (27).
c
The TTGE showed a pattern instead of a single band.
d
ND, not determined.
e
The subject showed two different L. crispatus strains.
f
The isolate has the same RAPD pattern as the type strain for L. jensenii.