TABLE 1.
S. schenckii isolates studied and their phenotypic characteristics
| Isolate origin, source, and isolate no.k | Conidial sizea (width × length) (μm) | Thermo- tolerance (% GIb) at:
|
LD50c (no. of conidia [107]/ml) | |
|---|---|---|---|---|
| 35°C | 37°C | |||
| MX, clinical | ||||
| EH-143,d Fe | 1.85 × 2.5 | 35.9 | 75.9 | 7.25 |
| EH-176, Ff | 2.4 × 2.9 | 23.1 | 65.2 | |
| EH-184, Lf | 2.5 × 2.6 | 47.1 | 68.7 | 135 |
| EH-189, Lg | 2.5 × 2.5 | 37.4 | 47.6 | 50 |
| EH-197, Fe | 1.5 × 1.6 | 29.4 | 54.6 | 237 |
| EH-198, Le | 2.1 × 2.1 | 33.9 | 56.5 | |
| EH-199, Le | 1.5 × 1.5 | 30.7 | 49.1 | |
| EH-200, Le | 1.4 × 1.5 | 24.0 | 57.5 | |
| EH-217, Fh | 1.7 × 1.7 | 50.4 | 63.1 | 7.0 |
| EH-218, Lh | 2.7 × 2.7 | 55.1 | 65.2 | |
| EH-220, Lh | 2.0 × 2.1 | 36.5 | 55.3 | |
| EH-221, Fh | 2.1 × 2.1 | 44.3 | 62.6 | |
| EH-241, Ff | 1.6 × 1.6 | 55.4 | 69.3 | |
| EH-247, Fh | 1.2 × 1.3 | 31.0 | 74.1 | 8.5 |
| EH-248, Lh | 1.2 × 1.3 | 46.0 | 66.4 | |
| EH-249, Lh | 1.2 × 1.3 | 49.0 | 67.6 | |
| EH-250, Fh | 2.5 × 2.5 | 42.2 | 66.7 | |
| MX, environmentale | ||||
| EH-193 | 1.8 × 1.8 | 30.9 | 45.9 | 6.7 |
| EH-194 | 1.5 × 1.5 | 24.8 | 24.7 | 1.4 |
| EH-195 | 1.3 × 1.4 | 33.2 | 54.3 | 2.5 |
| EH-251 | 2.7 × 2.8 | 47.2 | 70.1 | 12.0 |
| EH-252 | 1.6 × 1.6 | 40.9 | 61.2 | |
| EH-253 | 2.4 × 2.5 | 53.9 | 70.6 | |
| EH-254 | 1.3 × 1.3 | 24.5 | 61.2 | 29.5 |
| EH-255 | 1.2 × 1.2 | 28.9 | 63.4 | |
| EH-256 | 3.0 × 3.1 | 23.7 | 62.5 | |
| EH-257 | 3.0 × 3.2 | 23.6 | 66.6 | 5.0 |
| GT, clinicali | ||||
| EH-230, L | 3.0 × 3.0 | 16.4 | 69.1 | 2.4 |
| EH-232, F | 1.3 × 1.3 | 39.2 | 70.2 | |
| EH-233, L | 3.0 × 3.5 | 33.6 | 66.2 | 28.5 |
| EH-243, F | 2.5 × 3.0 | 18.2 | 70.7 | |
| EH-244, F | 3.0 × 3.1 | 24.7 | 77.0 | |
| EH-245, L | 3.5 × 3.8 | 29.3 | 69.8 | 24.5 |
| EH-246, L | 1.6 × 1.6 | 30.7 | 66.7 | 4.8 |
| CO, clinicalj | ||||
| 022, F | 1.6 × 4.1 | 51.8 | 63.7 | |
| 6067, F | 1.6 × 3.3 | 68.0 | 74.0 | 3.5 |
| 6514, L | 1.6 × 4.0 | 48.2 | 80.3 | 290 |
| 6780, F | 1.6 × 3.3 | 31.9 | 68.9 | 12.0 |
| 9362, L | 1.9 × 4.8 | 68.4 | 81.8 | |
| 10937; F | 1.9 × 4.1 | 30.8 | 70.9 | |
| 12926, F | 1.6 × 3.3 | 28.0 | 79.5 | |
| 15895, F | 1.9 × 3.8 | 71.0 | 74.5 | 170 |
| 9805079; F | 1.6 × 3.8 | 56.0 | 71.9 | |
| 9806027, L | 2.2 × 5.4 | 51.9 | 79.1 | 9.5 |
A minimum of 10 conidia from each isolate were grown on PDA plates for 15 days at 28°C, and measurements were made with a calibrated ocular.
Percent GIs were calculated in triplicate for samples from three different assays by the formula provided in Materials and Methods.
The LD50 was calculated for selected isolates from each geographical origin. For a better comparison of results, all LD50s were expressed as the number of conidia × 107 (same exponent).
Reference isolate.
From A. Espinosa Texis, Universidad Autónoma de Puebla, Puebla, Mexico.
From R. Arenas, Hospital Gea González, González, González, Mexico City, Mexico.
From A. Bonifaz, Hospital General de México, Mexico City, Mexico.
From D. Martinez, Instituto Dermatológico de Guadalajara, Guadalajara, Mexico.
From H. Logemann, Universidad de San Carlos de Guatemala. Guatemala City, Guatemala.
From A. Mesa Arango, Universidad de Antioquia, Medellín, Colombia.
F, fixed form of sporotrichosis; L, lymphocutaneous form of sporotrichosis.