Table 2. Results of ring DNA BBP graft-to synthesis.
| OC | SC | |||
|---|---|---|---|---|
| Equivalence | Apparent Mw a (kDa) | Graft densityb (%) | Apparent Mw a (kDa) | Graft densityb (%) |
| R pEYFP + mPEG750CEA | ||||
| 0 | 3627 | 0.0 | 1667 | 0.0 |
| 0.5 | 3663 | 0.8 | 1717 | 1.2 |
| 1 | 3735 | 2.5 | 1751 | 1.9 |
| 2.5 | 3847 | 5.1 | 1876 | 4.8 |
| 5 | 4081 | 10.5 | ||
| 10 | 4503 | 20.2 | ||
| 20 | 5323 | 39.1 | ||
| 40 | 6418 | 64.3 | ||
| R pEYFP + mPEG2kCEA | ||||
| 0 | 3589 | 0.0 | 1639 | 0.0 |
| 0.5 | 3743 | 1.4 | 1696 | 0.3 |
| 1 | 3828 | 2.2 | 1754 | 0.9 |
| 2.5 | 4026 | 4.1 | 1881 | 2.1 |
| 5 | 4430 | 7.8 | ||
| 10 | 5332 | 16.2 | ||
| 20 | 8691 | 47.3 | ||
| 40 | 10 519 | 64.3 | ||
| R pEYFP + mPEG5kCEA | ||||
| 0 | 3796 | 0.0 | 1710 | 0.0 |
| 0.5 | 4102 | 1.2 | 1852 | 0.5 |
| 1 | 4295 | 1.9 | 2139 | 1.7 |
| 2.5 | 5036 | 4.8 | 2395 | 2.6 |
| 5 | 5979 | 8.4 | 2947 | 4.8 |
| 10 | 8271 | 17.3 | ||
| 20 | 16 093 | 47.5 | ||
| 40 | 23 165 | 74.8 | ||
a
Apparent Mw calculated by extrapolation based on the 1 kb DNA ladder reference.
b
Grafting density calculated based on the difference in extrapolated Mw values for each DNA BBP conjugate.