Table 2.
Genes selected for validation of differential expression through Real-Time PCR.
| Gene ID | Gene Description | Gene Name | Forward Primer | Reverse Primer | Product Size (bp) |
|---|---|---|---|---|---|
| ENSSAUG00010018560/XM_030411990.1 | elongation factor 1-alpha, somatic form | ef1a | TCAAGGGATGGAAGGTTGAG | AGTTCCAATACCGCCGAT | 152 |
| ENSSAUG00010007010 | hemoglobin, beta adult 2 | hbba2 | GCAAGGGTGCTGATCGTCTA | GGGCTGCCACTTTGGAGTTA | 103 |
| ENSSAUG00010003114 | ribosomal protein L13a | rpl13 | TCTGGAGGACTGTCAGGGGCATGC | AGACGCACAATCTTAAGAGCAG | 197 |
| ENSSAUG00010000811 | 40S ribosomal protein S18 | rps18 | AGGGTGTTGGCAGACGTTAC | GAGGACCTGGCTGTATTTGC | 148 |
| ENSSAUG00010012992 | complement C1q-like protein 3 | c1ql3 | TTTGGAGACGGAGCGAAGAC | CCATACGCCTCACCACCTTT | 121 |
| ENSSAUG00010012990 | complement C1q-like protein 4 | c1ql4 | AGGTTGACACAGCCTTCCATA | CACTCATGTTGGGTTTGCAGG | 111 |
| ENSSAUG00010011851 | Carbonic anhydrase | cahz | AGGTGGACTTTGTGGACGAC | AAGCTCACAGGGGAACTTGA | 155 |
| ENSSAUG00010003856 | Rieske (Fe-S) domain containing | rfe.sdt | AGATGTGCATCGTTTGTCCA | TAGACATCCCCGTTGGTCTC | 166 |
| ENSSAUG00010006080 | NADH:ubiquinone oxidoreductase core subunit S1 | ndufs | CCCACTCTTCAACGCCAGAA | TCCCAGGTGGTCATACGAGT | 106 |
| ENSSAUG00010002150 | NADH:ubiquinone oxidoreductase subunit A2 | ndufa2 | CAGTAAGGGGGCCAGAGATT | GTTGTCCACCATGACACTGC | 157 |
| ENSSAUG00010006606 | mitochondrial ribosomal protein L11 | mrpl11 | ACGAGATCGCAAGGGTTAAA | GCTGCTCCAGGAAGATTTTG | 159 |
| ENSSAUG00010007642 | translocase of outer mitochondrial membrane 34 | tomm34 | CCTGTCGGTGAAGCAGTACA | AGGTTGTTCAGGTCGTCCAC | 150 |
| ENSSAUG00010025859 | heme oxygenase-like | hmo2 | CGCCTACACCCGTTATCTGG | GCTGTTCATCCTGCTCCTGT | 166 |
| ENSSAUG00010025665 | heat shock protein HSP 90-alpha | hsp90aa | TGACCCTCAGACACACTCCA | GTCGTCATCGTCCCCTTCAA | 139 |
| ENSSAUG00010000727 | major histocompatibility complex classI-related gene protein -like | mhc class 1 | AGATCGGATCGGAACCAACG | CGATGAATCCAACAGCACCG | 107 |