Figure 3.

Monitoring of the various welfare parameters on healthy mice (pre), during tumor progression (tp), and on the days (5–1) directly before or at the humane endpoint (hep), respectively, for the different therapy groups in an orthotopic pancreatic cancer model. The percentage of body weight change (A), the distress score (B), and perianal temperature (C) were monitored on the six days before and on the day of the hep (6, 5, 4, 3, 2, 1, and hep). The number of burrowed pellets after two hours (D) was analyzed for four days prior to hep and on the day of hep. The number of burrowed pellets after 17 h (E), nesting score (F), and MGS score (G) were analyzed for three days before and on the day of the hep. The statistical analysis was carried out with repeated measures two-way ANOVA (A,B), followed by Dunnett’s test for comparisons with tumor progression values within the models (# p ≤ 0.05) and Tukey’s test for inter-model differences. The grey area represents the baseline values for each parameter of all the mice before the tumor cell injection. Vehicle (n = 3–5); Erlotinib (n = 4–7); LXH254 (n = 3–6); Erlotinib + LXH254 (n = 4–7).